Optical measurement of cycle-dependent cell growth
Mustafa Mir,Zhuo Wang,Zhen Shen,Michael Bednarz,Rashid Bashir,Ido Golding,Supriya G. Prasanth,Gabriel Popescu +7 more
TLDR
It is demonstrated that a newly developed optical interferometric technique, known as spatial light interference microscopy, can measure the cell dry mass of many individual adherent cells in various conditions, over spatial scales from micrometers to millimeters, temporal scales ranging from seconds to days, and cell types ranging from bacteria to mammalian cells.Abstract:
Determining the growth patterns of single cells offers answers to some of the most elusive questions in contemporary cell biology: how cell growth is regulated and how cell size distributions are maintained. For example, a linear growth in time implies that there is no regulation required to maintain homeostasis; an exponential pattern indicates the opposite. Recently, there has been great effort to measure single cells using microelectromechanical systems technology, and several important questions have been explored. However, a unified, easy-to-use methodology to measure the growth rate of individual adherent cells of various sizes has been lacking. Here we demonstrate that a newly developed optical interferometric technique, known as spatial light interference microscopy, can measure the cell dry mass of many individual adherent cells in various conditions, over spatial scales from micrometers to millimeters, temporal scales ranging from seconds to days, and cell types ranging from bacteria to mammalian cells. We found evidence of exponential growth in Escherichia coli, which agrees very well with other recent reports. Perhaps most importantly, combining spatial light interference microscopy with fluorescence imaging provides a unique method for studying cell cycle-dependent growth. Thus, by using a fluorescent reporter for the S phase, we measured single cell growth over each phase of the cell cycle in human osteosarcoma U2OS cells and found that the G2 phase exhibits the highest growth rate, which is mass-dependent and can be approximated by an exponential.read more
Citations
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Quantitative phase imaging in biomedicine
TL;DR: This Review presents the main principles of operation and representative basic and clinical science applications of quantitative phase imaging, and aims to provide a critical and objective overview of this dynamic research field.
Spatial light interference microscopy (SLIM)
TL;DR: Spatial light interference microscopy reveals the intrinsic contrast of cell structures and renders quantitative optical path-length maps across the sample, which may prove instrumental in impacting the light microscopy field at a large scale.
Journal ArticleDOI
Quantitative phase imaging techniques for the study of cell pathophysiology: from principles to applications.
KyeoReh Lee,Kyoohyun Kim,JaeHwang Jung,Ji Han Heo,Sangyeon Cho,SangYun Lee,Gyuyoung Chang,YoungJu Jo,HyunJoo Park,YongKeun Park +9 more
TL;DR: The principles of QPI are presented and some of the recent applications ranging from cell homeostasis to infectious diseases and cancer are highlighted, to provide important insight on how the QPI techniques potentially improve the study of cell pathophysiology.
Journal ArticleDOI
Cell refractive index for cell biology and disease diagnosis: past, present and future
P. Y. Liu,P. Y. Liu,Lip Ket Chin,Wee Ser,H. F. Chen,Chao Mao Hsieh,Chau-Hwang Lee,Kung-Bin Sung,T. C. Ayi,Peng Huat Yap,Bo Liedberg,Kuan Wang,Kuan Wang,Tarik Bourouina,Yamin Leprince-Wang +14 more
TL;DR: An overview of cell refractive index models and measurement techniques including microfluidic chip-based techniques for the last 50 years are provided, the applications and significance of cellRefractive index in cell biology, hematology, and pathology are presented, and future research trends in the field are discussed.
Journal ArticleDOI
The regulation of cell size.
TL;DR: The current understanding of how a cell sets its size, how it can adapt its size to a changing environment, and how these processes are relevant to human disease are discussed.
References
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Heinrich Leonhardt,Hans-Peter Rahn,Peter Weinzierl,Anje Sporbert,Thomas Cremer,Daniele Zink,M. Cristina Cardoso +6 more
TL;DR: Four dimensional analyses suggested that replication factories are stably anchored in the nucleus and that changes in the pattern occur through gradual, coordinated, but asynchronous, assembly and disassembly throughout S phase.
Journal ArticleDOI
Spatial light interference microscopy (SLIM)
Zhuo Wang,Larry J. Millet,Mustafa Mir,Huafeng Ding,Sakulsuk Unarunotai,John A. Rogers,Martha U. Gillette,Gabriel Popescu +7 more
TL;DR: The spatial light interference microscopy (SLIM) as mentioned in this paper is a new optical microscopy technique, capable of measuring nanoscale structures and dynamics in live cells via interferometry.
Journal ArticleDOI
Optical imaging of cell mass and growth dynamics
Gabriel Popescu,Young Keun Park,Niyom Lue,Catherine Best-Popescu,Lauren P. Deflores,Ramachandra R. Dasari,Michael S. Feld,Kamran Badizadegan,Kamran Badizadegan +8 more
TL;DR: Overall, this study shows that interferometeric quantitative phase microscopy represents a noninvasive optical assay for monitoring cell growth, characterizing cellular motility, and investigating the subcellular motions of living cells.
Spatial light interference microscopy (SLIM)
TL;DR: Spatial light interference microscopy reveals the intrinsic contrast of cell structures and renders quantitative optical path-length maps across the sample, which may prove instrumental in impacting the light microscopy field at a large scale.
Journal ArticleDOI
Cell growth and size homeostasis in proliferating animal cells.
TL;DR: It is concluded that proliferating mammalian cells have an intrinsic mechanism that maintains cell size, and growth rate is size-dependent throughout the cell cycle.