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Isabel Moura

Researcher at Universidade Nova de Lisboa

Publications -  421
Citations -  14404

Isabel Moura is an academic researcher from Universidade Nova de Lisboa. The author has contributed to research in topics: Desulfovibrio gigas & Rubredoxin. The author has an hindex of 61, co-authored 420 publications receiving 13481 citations. Previous affiliations of Isabel Moura include University of Vigo & University of New Mexico.

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Conversion of [3 Fe3 S] into [4 Fe4 S] clusters in a Desulfovibrio gigas ferredoxin and isotopic labeling of iron—sulfur cluster subsites

TL;DR: In this paper, the authors proposed a method to solve the problem of protein synthesis in the context of bioinformatics and applied it in the field of bioengineering at the University of Minnesota.
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Spectroscopic Definition of the CuZ° Intermediate in Turnover of Nitrous Oxide Reductase and Molecular Insight into the Catalytic Mechanism

TL;DR: A lower limit on the rate of this intramolecular electron transfer (IET) that is >104 faster than the unobserved IET in the resting state is obtained, showing that CuZ° is the catalytically relevant oxidized form of N2OR.
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Electrochemical behaviour of bacterial nitric oxide reductase-evidence of low redox potential non-heme Fe(B) gives new perspectives on the catalytic mechanism.

TL;DR: The Pseudomonas nautica cNOR electrochemical behaviour was studied by cyclic voltammetry (CV), using a pyrolytic graphite electrode modified with the immobilised protein, and the protein redox centres were observed and the formal redox potentials were determined.
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Ca2+ and the bacterial peroxidases: the cytochrome c peroxidase from Pseudomonas stutzeri.

TL;DR: Molecular mass determination by ultracentrifugation and behavior on gel filtration chromatography have revealed that this CCP is isolated as an active dimer, in contrast to the Pa. denitrificans CCP which requires added Ca2+ for formation of the dimer and also for activation of the enzyme.
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Biosensor for direct bioelectrocatalysis detection of nitric oxide using nitric oxide reductase incorporated in carboxylated single-walled carbon nanotubes/lipidic 3 bilayer nanocomposite.

TL;DR: The proposed biosensor, by incorporating the benefits of biomimetic features of the phospholipid bilayer with SWCNT's inherent properties and NOR bioelectrocatalytic activity and selectivity, is a promising tool for NO.