J. Andrew Jones

TL;DR: This work discusses cell physiological responses to metabolic burdens, as well as strategies to identify and resolve the carbon and energy burden problems, including metabolic balancing, enhancing respiration, dynamic regulatory systems, chromosomal engineering, decoupling cell growth with production phases, and co- utilization of nutrient resources.

TL;DR: This study presents an Escherichia coli co-culture for the efficient production of flavonoids in vivo, resulting in a 970-fold improvement in titer of flavan-3-ols over previously published monoculture production.

TL;DR: This review highlights up-to-date case studies using commonly used tools and universalized methodologies for metabolic pathway balancing and optimization, and examines their potential and importance for production of compounds such as fatty acids, alcohols, and high value chemicals.

TL;DR: This work engineer for the first time the complex microbial biosynthesis of an anthocyanin plant natural product, starting from sugar, through the development of a synthetic, 4-strain Escherichia coli polyculture collectively expressing 15 exogenous or modified pathway enzymes from diverse plants and other microbes.

TL;DR: By incorporating the pathway to synthesize the flavanone naringenin, the first example of in vivo conversion of methanol into a specialty chemical in E. coli is demonstrated, demonstrating a cycling RuMP pathway for meethanol assimilation to support growth.