J
James M. Carothers
Researcher at University of Washington
Publications - 43
Citations - 2879
James M. Carothers is an academic researcher from University of Washington. The author has contributed to research in topics: Aptamer & Synthetic biology. The author has an hindex of 17, co-authored 37 publications receiving 2476 citations. Previous affiliations of James M. Carothers include Harvard University & California Institute for Quantitative Biosciences.
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Journal ArticleDOI
Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids.
TL;DR: This DSRS substantially improved the stability of biodiesel-producing strains and increased the titer and yield threefold and can be extended to many other biosynthetic pathways to balance metabolism, thereby increasing product titers and conversion yields and stabilizing production hosts.
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ECSIT is an evolutionarily conserved intermediate in the Toll/IL-1 signal transduction pathway
Elizabeth Kopp,Ruslan Medzhitov,James M. Carothers,Changchun Xiao,Iris Douglas,Charles A. Janeway,Sankar Ghosh +6 more
TL;DR: It is suggested that processing of MEKK-1 is required for its function in the Toll/IL-1 pathway and an important role for ECSIT in signaling to NF-kappaB is indicated.
Journal ArticleDOI
Informational complexity and functional activity of RNA structures.
TL;DR: A comparison of the structures and activities of eleven distinct GTP-binding RNAs (aptamers) is presented, showing that defining a structure capable of 10-fold tighter binding requires approximately 10 additional bits of information.
Journal ArticleDOI
Model-Driven Engineering of RNA Devices to Quantitatively Program Gene Expression
James M. Carothers,Jonathan A. Goler,Darmawi Juminaga,Jay D. Keasling,Jay D. Keasling,Jay D. Keasling +5 more
TL;DR: A design-driven approach was formulated that used mechanistic modeling and kinetic RNA folding simulations to engineer RNA-regulated genetic devices that control gene expression, and the potential for the use of biochemical and biophysical modeling to develop biological design methods was illustrated.
Journal ArticleDOI
Measurement and modeling of intrinsic transcription terminators.
Guillaume Cambray,Joao C. Guimaraes,Vivek K. Mutalik,Colin Lam,Quynh-Anh Mai,Tim Thimmaiah,James M. Carothers,Adam P. Arkin,Drew Endy +8 more
TL;DR: A genetic architecture is developed and validated that enables reliable measurement of termination efficiencies and it is found that structures extending beyond the core terminator stem are likely to increase terminator activity.