Jan-Willem de Gier

TL;DR: A derivative strain of E. coli BL21(DE3) is engineered, termed Lemo21(de3), in which the activity of the T7 RNA polymerase can be precisely controlled by its natural inhibitor T7 lysozyme (T7Lys), which allows optimizing overexpression of any given membrane protein by using only a single strain rather than a multitude of different strains.

TL;DR: It is proposed that YidC, homologous to Saccharomyces cerevisiae Oxa1p, is involved in the insertion of hydrophobic sequences into the lipid bilayer after initial recognition by the SecAYEG translocase.

TL;DR: In this protocol the Escherichia coli BL21(DE3)-pET system—the most widely used (membrane) protein overexpression system—is used as a platform to illustrate the GFP-based method and the methodology described in this protocol can be transferred easily to other systems.

TL;DR: It is demonstrated here that the SRP pathway delivers nascent inner membrane proteins at the membrane through a protein cross‐linking approach that delivers a variety of precursor proteins to a common membrane translocation complex of the E. coli inner membrane.

TL;DR: Induction of the acetate-phosphotransacetylase pathway for ATP production and a down-regulated tricarboxylic acid cycle indicated the activation of the Arc two-component system, which mediates adaptive responses to changing respiratory states.