J
Janice S. Chen
Researcher at University of California, Berkeley
Publications - 22
Citations - 5413
Janice S. Chen is an academic researcher from University of California, Berkeley. The author has contributed to research in topics: Cas9 & CRISPR. The author has an hindex of 14, co-authored 22 publications receiving 3118 citations. Previous affiliations of Janice S. Chen include Lawrence Berkeley National Laboratory & University of California.
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Journal ArticleDOI
CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
Janice S. Chen,Enbo Ma,Lucas B. Harrington,Maria Da Costa,Xinran Tian,Joel M. Palefsky,Jennifer A. Doudna +6 more
TL;DR: It is shown that RNA-guided DNA binding unleashes indiscriminate single-stranded DNA cleavage activity by Cas12a that completely degrades ssDNA molecules, which is also a property of other type V CRISPR-Cas12 enzymes.
Journal ArticleDOI
Enhanced proofreading governs CRISPR–Cas9 targeting accuracy
Janice S. Chen,Yavuz S. Dagdas,Benjamin P. Kleinstiver,Moira M. Welch,Alexander A. Sousa,Lucas B. Harrington,Samuel H. Sternberg,J. Keith Joung,Ahmet Yildiz,Jennifer A. Doudna +9 more
TL;DR: A new hyper-accurate Cas9 variant (HypaCas9) is designed that demonstrates high genome-wide specificity without compromising on-target activity in human cells and offers a more comprehensive model to rationalize and modify the balance between target recognition and nuclease activation for precision genome editing.
Journal ArticleDOI
Programmed DNA destruction by miniature CRISPR-Cas14 enzymes.
Lucas B. Harrington,David Burstein,Janice S. Chen,David Paez-Espino,Enbo Ma,Isaac P. Witte,Joshua C Cofsky,Nikos C. Kyrpides,Jillian F. Banfield,Jennifer A. Doudna +9 more
TL;DR: Metagenomic data show that multiple CRISPR-Cas14 systems evolved independently and suggest a potential evolutionary origin of single-effector CRISpr-based adaptive immunity, as well as a fast and high-fidelity nucleic acid detection system that enabled detection ofsingle-nucleotide polymorphisms.
Journal ArticleDOI
Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage.
Fuguo Jiang,David W. Taylor,Janice S. Chen,Jack E. Kornfeld,Kaihong Zhou,Aubri J. Thompson,Eva Nogales,Jennifer A. Doudna +7 more
TL;DR: Molecular structures of the catalytically active Streptococcus pyogenes Cas9 R-loop are determined that show the displaced DNA strand located near the RuvC nuclease domain active site in a conformation essential for concerted DNA cutting.
Posted ContentDOI
Enhanced proofreading governs CRISPR-Cas9 targeting accuracy
Janice S. Chen,Yavuz S. Dagdas,Benjamin P. Kleinstiver,Moira M. Welch,Lucas B. Harrington,Samuel H. Sternberg,J. Keith Joung,Ahmet Yildiz,Jennifer A. Doudna +8 more
TL;DR: A non-catalytic domain within Cas9, REC3, recognizes target mismatches and governs the HNH nuclease to regulate overall catalytic competence, and a new hyper-accurate Cas9 variant (HypaCas9) is designed that retains robust on-target activity in human cells.