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Lucas B. Harrington
Researcher at University of California
Publications - 29
Citations - 5727
Lucas B. Harrington is an academic researcher from University of California. The author has contributed to research in topics: CRISPR & Cas9. The author has an hindex of 17, co-authored 29 publications receiving 3432 citations. Previous affiliations of Lucas B. Harrington include University of California, Berkeley.
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Journal ArticleDOI
CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
Janice S. Chen,Enbo Ma,Lucas B. Harrington,Maria Da Costa,Xinran Tian,Joel M. Palefsky,Jennifer A. Doudna +6 more
TL;DR: It is shown that RNA-guided DNA binding unleashes indiscriminate single-stranded DNA cleavage activity by Cas12a that completely degrades ssDNA molecules, which is also a property of other type V CRISPR-Cas12 enzymes.
Journal ArticleDOI
Enhanced proofreading governs CRISPR–Cas9 targeting accuracy
Janice S. Chen,Yavuz S. Dagdas,Benjamin P. Kleinstiver,Moira M. Welch,Alexander A. Sousa,Lucas B. Harrington,Samuel H. Sternberg,J. Keith Joung,Ahmet Yildiz,Jennifer A. Doudna +9 more
TL;DR: A new hyper-accurate Cas9 variant (HypaCas9) is designed that demonstrates high genome-wide specificity without compromising on-target activity in human cells and offers a more comprehensive model to rationalize and modify the balance between target recognition and nuclease activation for precision genome editing.
Journal ArticleDOI
Programmed DNA destruction by miniature CRISPR-Cas14 enzymes.
Lucas B. Harrington,David Burstein,Janice S. Chen,David Paez-Espino,Enbo Ma,Isaac P. Witte,Joshua C Cofsky,Nikos C. Kyrpides,Jillian F. Banfield,Jennifer A. Doudna +9 more
TL;DR: Metagenomic data show that multiple CRISPR-Cas14 systems evolved independently and suggest a potential evolutionary origin of single-effector CRISpr-based adaptive immunity, as well as a fast and high-fidelity nucleic acid detection system that enabled detection ofsingle-nucleotide polymorphisms.
Journal ArticleDOI
New CRISPR–Cas systems from uncultivated microbes
David Burstein,Lucas B. Harrington,Steven C. Strutt,Alexander J. Probst,Karthik Anantharaman,Brian C. Thomas,Jennifer A. Doudna,Jillian F. Banfield +7 more
TL;DR: A number of CRISPR–Cas systems, including the first reported Cas9 in the archaeal domain of life, are identified using genome-resolved metagenomics, enabling validation of robust in vivo RNA-guided DNA interference activity in Escherichia coli.
Journal ArticleDOI
CasX enzymes comprise a distinct family of RNA-guided genome editors
Jun-Jie Liu,Jun-Jie Liu,Jun-Jie Liu,Natalia Orlova,Benjamin L. Oakes,Enbo Ma,Hannah Spinner,Katherine Baney,Jonathan Chuck,Dan Tan,Gavin J. Knott,Lucas B. Harrington,Basem Al-Shayeb,Alexander J. Wagner,Julian Brötzmann,Brett T. Staahl,Kian Taylor,John J Desmarais,Eva Nogales,Jennifer A. Doudna +19 more
TL;DR: The underlying mechanisms of a third, fundamentally distinct RNA-guided genome-editing platform named CRISPR–CasX, which uses unique structures for programmable double-stranded DNA binding and cleavage, are revealed.