Jarrett D. Egertson

TL;DR: The ProteoWizard Toolkit is developed, a robust set of open-source, software libraries and applications designed to facilitate proteomics research that implements the first-ever, non-commercial, unified data access interface for proteomics, bridging field-standard open formats and all common vendor formats.

TL;DR: The modified molecular inversion probe method was applied to 44 candidate genes to identify de novo mutations in a large cohort of individuals with and without autism spectrum disorder, supporting the notion that multiple genes underlie autism-spectrum disorders.

TL;DR: It is found that by exploiting calibrated retention time and fragmentation specificity in chromatogram libraries, EncyclopeDIA can detect 20–25% more peptides from DIA experiments than with data dependent acquisition-based spectrum libraries alone.

TL;DR: A multiplexing strategy is demonstrated for DIA analysis that increases precursor selectivity fivefold and can be used for data-dependent acquisition or selected reaction monitoring.

TL;DR: The use of data-independent acquisition (DIA) on a Q-Exactive mass spectrometer for the detection and quantification of peptides in complex mixtures using the Skyline Targeted Proteomics Environment is described.