J
Jason Gibson
Publications - 1
Citations - 19
Jason Gibson is an academic researcher. The author has contributed to research in topics: Cas9 & Genome editing. The author has an hindex of 1, co-authored 1 publications receiving 1 citations.
Papers
More filters
Journal ArticleDOI
Conformational control of Cas9 by CRISPR hybrid RNA-DNA guides mitigates off-target activity in T cells
Paul Daniel Donohoue,Martin Pacesa,Elaine Lau,Bastien Vidal,Matthew J. Irby,David B. Nyer,Tomer Rotstein,Lynda M. Banh,Mckenzi S. Toh,Jason Gibson,B. Kohrs,Kevin Baek,Arthur L.G. Owen,Euan M. Slorach,Megan van Overbeek,Chris R. Fuller,Andrew May,Martin Jinek,Peter Cameron +18 more
TL;DR: In this article, a CRISPR hybrid RNA-DNA (chRDNA) guides were used to increase Cas9 specificity while preserving on-target editing activity, by perturbing DNA hybridization and modulating Cas9 activation kinetics to disfavor binding and cleavage of off target substrates.