M
Matthew J. Irby
Publications - 5
Citations - 108
Matthew J. Irby is an academic researcher. The author has contributed to research in topics: CRISPR & Cas9. The author has an hindex of 1, co-authored 4 publications receiving 36 citations.
Papers
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Journal ArticleDOI
Harnessing type I CRISPR–Cas systems for genome engineering in human cells
Peter Cameron,Mary M. Coons,Sanne E. Klompe,Alexandra M. Lied,Stephen C. Smith,Bastien Vidal,Paul Daniel Donohoue,Tomer Rotstein,B. Kohrs,David B. Nyer,Rachel Kennedy,Lynda M. Banh,Carolyn Williams,Mckenzi S. Toh,Matthew J. Irby,Leslie S. Edwards,Chun Han Lin,Arthur L.G. Owen,Tim Künne,John van der Oost,Stan J. J. Brouns,Stan J. J. Brouns,Euan M. Slorach,Chris R. Fuller,Scott Gradia,Steven B. Kanner,Andrew May,Samuel H. Sternberg +27 more
TL;DR: This work demonstrates that highly abundant, previously untapped type I CRISPR–Cas systems can be harnessed for genome engineering applications in eukaryotic cells.
Journal ArticleDOI
Conformational control of Cas9 by CRISPR hybrid RNA-DNA guides mitigates off-target activity in T cells
Paul Daniel Donohoue,Martin Pacesa,Elaine Lau,Bastien Vidal,Matthew J. Irby,David B. Nyer,Tomer Rotstein,Lynda M. Banh,Mckenzi S. Toh,Jason Gibson,B. Kohrs,Kevin Baek,Arthur L.G. Owen,Euan M. Slorach,Megan van Overbeek,Chris R. Fuller,Andrew May,Martin Jinek,Peter Cameron +18 more
TL;DR: In this article, a CRISPR hybrid RNA-DNA (chRDNA) guides were used to increase Cas9 specificity while preserving on-target editing activity, by perturbing DNA hybridization and modulating Cas9 activation kinetics to disfavor binding and cleavage of off target substrates.
Posted ContentDOI
Structural basis for Cas9 off-target activity
Martin Pacesa,Chun Han Lin,Antoine Cléry,Katja Bargsten,Matthew J. Irby,Frédéric H.-T. Allain,Peter Cameron,Paul Daniel Donohoue,Martin Jinek +8 more
TL;DR: In this paper, the authors report crystallographic structures of Cas9 bound to bona fide off-target substrates, revealing that offtarget binding is enabled by a range of non-canonical base pairing interactions and preservation of base stacking within the guide-off-target heteroduplex.
Journal ArticleDOI
Allogeneic chimeric antigen receptor-T cells with CRISPR-disrupted programmed death-1 checkpoint exhibit enhanced functional fitness.
Elaine Lau,George Kwong,Tristan Fowler,Bee Sun,Paul Daniel Donohoue,Ryan T. Davis,Mara Bryan,Starlynn Clarke,Carolyn Williams,Lynda M. Banh,Matthew J. Irby,Leslie S. Edwards,Meghan D. Storlie,B. Kohrs,Graham W. G. Lilley,Stephen C. Smith,Scott Gradia,Chris R. Fuller,Justin Skoble,E. Garner,Megan van Overbeek,Steven B. Kanner +21 more
TL;DR: In this paper , CAR-T cells (CB-010) were genome edited with a next-generation CRISPR-based platform (Cas9 chRDNA) by knockout of the PDCD1 gene encoding the PD-1 receptor.
Patent
Crispr abasic restricted nucleotides and crispr accuracy via analogs
TL;DR: In this article, the authors provide polynucleotide guides for use in CRISPR-Cas systems, where polynotides contain at least one CRisPR abasic restricted nucleotide (CABRNT) and/or at least 1 CAVA nucleotide.