B
Bastien Vidal
Publications - 4
Citations - 782
Bastien Vidal is an academic researcher. The author has contributed to research in topics: Cas9 & Genome editing. The author has an hindex of 3, co-authored 4 publications receiving 529 citations.
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Journal ArticleDOI
DNA Repair Profiling Reveals Nonrandom Outcomes at Cas9-Mediated Breaks
Megan van Overbeek,Daniel Capurso,Matthew Merrill Carter,Matthew S. Thompson,Elizabeth Frias,Carsten Russ,John S. Reece-Hoyes,Christopher D. Nye,Scott Gradia,Bastien Vidal,Jiashun Zheng,Gregory R. Hoffman,Chris R. Fuller,Andrew May +13 more
TL;DR: This work demonstrates that the pattern of DNA repair following Cas9 cutting at each site is nonrandom and consistent across experimental replicates, cell lines, and reagent delivery methods, and elucidates a strategy for using "error-prone" DNA-repair machinery to generate precise edits.
Journal ArticleDOI
Mapping the genomic landscape of CRISPR–Cas9 cleavage
Cameron Peter Sean,Chris R. Fuller,Paul Daniel Donohoue,Brittnee N. Jones,Matthew S. Thompson,Matthew Merrill Carter,Scott Gradia,Bastien Vidal,Elizabeth Garner,Euan M. Slorach,Elaine Lau,Lynda M. Banh,Alexandra M. Lied,Leslie S. Edwards,Alexander H. Settle,Daniel Capurso,Victor Llaca,Stéphane Deschamps,A. Mark Cigan,Joshua K. Young,Andrew May +20 more
TL;DR: This work developed a biochemical method (SITE-Seq), using Cas9 programmed with single-guide RNAs (sgRNAs), to identify the sequence of cut sites within genomic DNA, and found that the number of sites identified depended on sgRNA sequence and nuclease concentration.
Journal ArticleDOI
Harnessing type I CRISPR–Cas systems for genome engineering in human cells
Peter Cameron,Mary M. Coons,Sanne E. Klompe,Alexandra M. Lied,Stephen C. Smith,Bastien Vidal,Paul Daniel Donohoue,Tomer Rotstein,B. Kohrs,David B. Nyer,Rachel Kennedy,Lynda M. Banh,Carolyn Williams,Mckenzi S. Toh,Matthew J. Irby,Leslie S. Edwards,Chun Han Lin,Arthur L.G. Owen,Tim Künne,John van der Oost,Stan J. J. Brouns,Stan J. J. Brouns,Euan M. Slorach,Chris R. Fuller,Scott Gradia,Steven B. Kanner,Andrew May,Samuel H. Sternberg +27 more
TL;DR: This work demonstrates that highly abundant, previously untapped type I CRISPR–Cas systems can be harnessed for genome engineering applications in eukaryotic cells.
Journal ArticleDOI
Conformational control of Cas9 by CRISPR hybrid RNA-DNA guides mitigates off-target activity in T cells
Paul Daniel Donohoue,Martin Pacesa,Elaine Lau,Bastien Vidal,Matthew J. Irby,David B. Nyer,Tomer Rotstein,Lynda M. Banh,Mckenzi S. Toh,Jason Gibson,B. Kohrs,Kevin Baek,Arthur L.G. Owen,Euan M. Slorach,Megan van Overbeek,Chris R. Fuller,Andrew May,Martin Jinek,Peter Cameron +18 more
TL;DR: In this article, a CRISPR hybrid RNA-DNA (chRDNA) guides were used to increase Cas9 specificity while preserving on-target editing activity, by perturbing DNA hybridization and modulating Cas9 activation kinetics to disfavor binding and cleavage of off target substrates.