Showing papers by "Javier M. Rodríguez published in 2008"
TL;DR: In ASFV-infected cells, the p10 protein strongly accumulates in the nucleus at late times post-infection, indicating that p10protein may accomplish an important function inside the nucleus during the late phase of the viral replication cycle.
14 citations
TL;DR: Using two-dimensional gel electrophoresis followed by MALDI-TOF to identify and purify heat shock protein HSP60A of Drosophila melanoagaster shows that it is heat-shock inducible and describes two novel antisera, specifically designed to recognize the denatured and native polypeptide, respectively, in Dosophila.
Abstract: The evolutionarily conserved hsp60 (heat-shock protein 60) family of molecular chaperones ensures the correct folding of nuclear-encoded proteins after their translocation across the mitochondrial membrane during development as well as after heat-shock treatment. Although the overexpression of HSP60 proteins and their localization in the cytoplasm have been linked with many humans pathologies, the detailed pattern of their expression in different animal models and their subcellular localization during normal development and in stress conditions are little-known. In this report, we have used two-dimensional gel electrophoresis followed by MALDI-TOF to identify and purify heat shock protein HSP60A of Drosophila melanoagaster. We demonstrate that it is heat-shock inducible and describe two novel antisera, specifically designed to recognize the denatured and native polypeptide, respectively, in Drosophila. Immunoelectron microscopy and immunostaining of Drosophila cells with these antibodies reveals that HSP6...
12 citations