S
Stephen J. Benkovic
Researcher at Pennsylvania State University
Publications - 696
Citations - 44847
Stephen J. Benkovic is an academic researcher from Pennsylvania State University. The author has contributed to research in topics: DNA clamp & DNA replication. The author has an hindex of 95, co-authored 689 publications receiving 42476 citations. Previous affiliations of Stephen J. Benkovic include University of Pennsylvania & Scripps Health.
Papers
More filters
Journal ArticleDOI
Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda
William D. Huse,Lakshmi Sastry,Sheila A. Iverson,Angray S. Kang,Angray S. Kang,Michelle A. Alting-Mees,Dennis R. Burton,Dennis R. Burton,Stephen J. Benkovic,Stephen J. Benkovic,Richard A. Lerner +10 more
TL;DR: A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire, which allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation.
Journal ArticleDOI
Assembly of combinatorial antibody libraries on phage surfaces: the gene III site.
TL;DR: A phagemid system was developed for the monovalent display of combinatorial antibody Fab libraries on the surface of filamentous phage M13, and may replace current antibody cloning techniques.
Journal ArticleDOI
A Perspective on Enzyme Catalysis
TL;DR: A case study for the enzyme dihydrofolate reductase provides evidence for coupled networks of predominantly conserved residues that influence the protein structure and motion that have important implications for the origin and evolution of enzymes, as well as for protein engineering.
Journal ArticleDOI
Colloidal Au-Enhanced Surface Plasmon Resonance for Ultrasensitive Detection of DNA Hybridization
Lin He,Michael D. Musick,Sheila R. Nicewarner,Frank Salinas,Stephen J. Benkovic,Michael J. Natan,Christine D. Keating +6 more
TL;DR: A new approach to ultrasensitive detection of DNA hybridization based on nanoparticle-amplified surface plasmon resonance (SPR) is described, with a greater than 10-fold increase in angle shift and a more than 1000-fold improvement in sensitivity for the target oligonucleotide as compared to the unamplify binding event.
Journal ArticleDOI
On-chip manipulation of single microparticles, cells, and organisms using surface acoustic waves
Xiaoyun Ding,Sz-Chin Steven Lin,Brian Kiraly,Hongjun Yue,Sixing Li,I-Kao Chiang,Jinjie Shi,Stephen J. Benkovic,Tony Jun Huang +8 more
TL;DR: Standing surface acoustic wave based “acoustic tweezers” are demonstrated that can trap and manipulate single microparticles, cells, and entire organisms in a single-layer microfluidic chip and will become a powerful tool for many disciplines of science and engineering.