Jay R. Greenberg

TL;DR: This work has measured the stability of mRNA in exponentially growing mouse L-cells without having to resort to the use of inhibitors of RNA synthesis, and found that poly A-containing mRNA has a half-life of 10 h, and thus turns over approximately once per cell generation (15 h).

TL;DR: It is concluded that most mRNA in polyribosomes is in close contact with proteins, and that histone mRNA can be crosslinked to many fewer proteins that most other mRNAs.

TL;DR: It is suggested that newly synthesized mRNA molecules emerging from the nucleus into the cytoplasm shed the proteins with which they were associated in the nucleus and become associated with a new set of proteins derived from the cytosol.

TL;DR: A set of proteins crosslinked to L-cell mRNA by irradiating polyribosomes with 254 nm ultraviolet light has been identified and Digestions with specific RNAases and chromatography on oligo(dT)-cellulose were used to show that a protein of 78,000 daltons was the only one crosslink to poly(A), and the other proteins were cross linked to sequences other than poly( A).

TL;DR: It is concluded that poly(A)-containing mRNA and non-histone poly( A)-lacking mRNA exist in polyribosomes in the form of ribonucleoprotein particles.