Showing papers by "Jean E. Schwarzbauer published in 2004"
TL;DR: How the extracellular matrix changes during the stages of tissue repair, how matricellular proteins affect cell-extracllular matrix interactions, and how these proteins might be exploited for use therapeutically are discussed.
867 citations
TL;DR: It is reported that syndecan-4 is essential for optimal fibroblast response to the three-dimensional fibrin-fibronectin provisional matrix that is deposited upon tissue injury.
Abstract: Syndecan-4 is a ubiquitously expressed heparan sulfate proteoglycan that modulates cell interactions with the extracellular matrix. It is transiently up-regulated during tissue repair by cells that mediate wound healing. Here, we report that syndecan-4 is essential for optimal fibroblast response to the three-dimensional fibrin-fibronectin provisional matrix that is deposited upon tissue injury. Interference with syndecan-4 function inhibits matrix contraction by preventing cell spreading, actin stress fiber formation, and activation of focal adhesion kinase and RhoA mediated-intracellular signaling pathways. Tenascin-C is an extracellular matrix protein that regulates cell response to fibronectin within the provisional matrix. Syndecan-4 is also required for tenascin-C action. Inhibition of syndecan-4 function suppresses tenascin-C activity and overexpression of syndecan-4 circumvents the effects of tenascin-C. In this way, tenascin-C and syndecan-4 work together to control fibroblast morphology and signaling and regulate events such as matrix contraction that are essential for efficient tissue repair.
134 citations
TL;DR: Surfaces modified with RGDC were shown to be effective for osteoblast binding and proliferation.
Abstract: α,ω-Diphosphonic acids self-assemble on the native oxide surfaces of Ti or Ti-6Al-4V. Heating gives strongly bonded phosphonate monolayers. Infrared and X-ray spectroscopic and water contact angle data show that the films are bonded to the surface by one phosphonate unit; the other remains a phosphonic acid. Surface loadings were measured by quartz crystal microbalance procedures. Mechanical shear strengths for the films were also measured; these do not correlate simply with surface loadings. Films formed from 1,12-diphosphonododecane were treated with zirconium tetra(tert-butoxide) to give surface Zr complex species; derivatives of these surface complexes are stable to hydrolysis under physiological conditions and are mechanically strong. The complexation reaction can be accomplished over the entire surface; alternatively, dropwise application of the alkoxide to the surface enables spatial control of deposition. The cell attractive peptide derivative RGDC can be bound to these surface Zr alkoxide complex...
84 citations
TL;DR: A new method is described to attach biological molecules to the surface of silicon using a self-assembled film of an organophosphonic acid bonded to the native or synthesized oxide-coated Si surface as a film of the corresponding phosphonate.
Abstract: A new method is described to attach biological molecules to the surface of silicon. Semiconductors such as Si modified with surface-bound capture molecules have enormous potential for use in biosensors for which an ideal detection platform should be inexpensive, recognize targets rapidly with high sensitivity and specificity, and possess superior stability. In this process, a self-assembled film of an organophosphonic acid is bonded to the native or synthesized oxide-coated Si surface as a film of the corresponding phosphonate. The phosphonate film is functionalized to enable covalently coupling biological molecules, ranging in size from small peptides to large multi-subunit proteins, to the Si surface. Surface modification and biomolecule coupling procedures are easily accomplished: all reactions can proceed in air, and most take place under ambient conditions. The biomolecule-modified surfaces are stable under physiological conditions, are selective for adhesion of specific cells types, and are reusable.
63 citations
TL;DR: In this unit, basic methods of detection, quantification, and visualization of fibrillar fibronectin matrix are described and differential solubility in the detergent deoxycholate is the principle for biochemical fractionation of fibronected matrix.
Abstract: The extracellular matrix acts as a framework for tissue architecture and dynamically regulates many cellular functions. Fibronectin is a ubiquitous extracellular matrix component that plays critical roles in matrix structure and in directing cell behaviors. Fibronectin is synthesized and secreted by many cell types including fibroblasts, endothelial cells, myoblasts, and astrocytes. Upon secretion, cells assemble fibronectin into a fibrillar network. During assembly, fibronectin is initially organized into fine cell-associated fibrils and, through continued accumulation of fibronectin, these fibrils are converted into a dense network of detergent-insoluble fibrils. Differential solubility in the detergent deoxycholate is the principle for biochemical fractionation of fibronectin matrix. Fibril assembly and organization can also be examined by immunofluorescence staining. In this unit, basic methods of detection, quantification, and visualization of fibrillar fibronectin matrix are described.
Keywords:
fibronectin;
extracellular matrix;
fibroblasts;
immunofluorescence;
quantification;
detergent extracts
55 citations
TL;DR: Findings provide novel mechanistic insights into this process by demonstrating that talin, a protein that links integrins to actin, is necessary for the inside-out activation of Integrin transmembrane receptors.
36 citations
TL;DR: This work has shown that alterations of the extracellular matrix component fibulin-1 rescue gonadogenesis in Caenorhabditis elegans in the absence of these proteases is a critical step toward understanding the role of extrace cellular matrix in organogenesis.
3 citations