Jennifer M. Clyne

TL;DR: The enzyme modified oligonucleotides were found to be significantly better labeling materials than the fluorescent or chemiluminescent derivatives, providing sensitivities comparable to 32P-labeled probes.

TL;DR: The detection of a little as 0.2 pg (60,000 molecules) of hepatitis B viral DNA in human serum samples in 4 h has been demonstrated using a solution-hybridization and bead-capture method and only in the presence of the virus was label specifically bound to the support.

TL;DR: Amplified solution-phase sandwich nucleic acid hybridization assays for HBV in which analyte is hybridized in solution with sets of amplifier probe oligonucleotides as mentioned in this paper.

TL;DR: Linear or branched oligonucleotide multimers are useful as amplifiers in biochemical assays which comprise (1) at least one first single-stranded oligonotide unit that is complementary to a singlestranded sequence of interest, and (2) a multiplicity of second single strand-linked oligon nucleotide units that are complementary to the label of interest as mentioned in this paper.

TL;DR: Linear or branched oligonucleotide multimers are useful as amplifiers in biochemical assays which comprise (1) at least one first single-stranded oligonotide unit that is complementary to a singlestranded sequence of interest, and (2) a multiplicity of second single strand-linked oligon nucleotide units that are complementary to the label of interest.