J
Jennifer M. Clyne
Researcher at Chiron Corporation
Publications - 13
Citations - 519
Jennifer M. Clyne is an academic researcher from Chiron Corporation. The author has contributed to research in topics: Oligonucleotide & Nucleic acid. The author has an hindex of 6, co-authored 13 publications receiving 518 citations. Previous affiliations of Jennifer M. Clyne include Siemens.
Papers
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Journal ArticleDOI
A comparison of non-radioisotopic hybridization assay methods using fluorescent, chemiluminescent and enzyme labeled synthetic oligodeoxyribonucleotide probes.
Mickey S. Urdea,Brian D. Warner,Joyce A. Running,Michelle M. Stempien,Jennifer M. Clyne,Thomas Horn +5 more
TL;DR: The enzyme modified oligonucleotides were found to be significantly better labeling materials than the fluorescent or chemiluminescent derivatives, providing sensitivities comparable to 32P-labeled probes.
Journal ArticleDOI
A novel method for the rapid detection of specific nucleotide sequences in crude biological samples without blotting or radioactivity; application to the analysis of hepatitis B virus in human serum
TL;DR: The detection of a little as 0.2 pg (60,000 molecules) of hepatitis B viral DNA in human serum samples in 4 h has been demonstrated using a solution-hybridization and bead-capture method and only in the presence of the virus was label specifically bound to the support.
Patent
Improved amplified nucleic acid hybridization assays for hbv.
Michael S. Urdea,Warner Brian D,Joyce A. Running,Janice A. Kolberg,Jennifer M. Clyne,Ray Sanchez-Pescador,Thomas Horn +6 more
TL;DR: Amplified solution-phase sandwich nucleic acid hybridization assays for HBV in which analyte is hybridized in solution with sets of amplifier probe oligonucleotides as mentioned in this paper.
Patent
Nucleic acid hybridization assay for hepatitis B virus DNA
Michael S. Urdea,Warner Brian D,Joyce A. Running,Janice A. Kolberg,Jennifer M. Clyne,Ray Sanchez-Pescador,Thomas Horn +6 more
TL;DR: Linear or branched oligonucleotide multimers are useful as amplifiers in biochemical assays which comprise (1) at least one first single-stranded oligonotide unit that is complementary to a singlestranded sequence of interest, and (2) a multiplicity of second single strand-linked oligon nucleotide units that are complementary to the label of interest as mentioned in this paper.
Patent
Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers
Michael S. Urdea,Warner Brian D,Joyce A. Running,Janice A. Kolberg,Jennifer M. Clyne,Ray Sanchez-Pescador,Thomas Horn +6 more
TL;DR: Linear or branched oligonucleotide multimers are useful as amplifiers in biochemical assays which comprise (1) at least one first single-stranded oligonotide unit that is complementary to a singlestranded sequence of interest, and (2) a multiplicity of second single strand-linked oligon nucleotide units that are complementary to the label of interest.