Jennifer M. Heemstra

TL;DR: Glyoxal modification is a straightforward and scarless method for imparting reversible thermal responsiveness to theoretically any nucleic acid architecture, addressing a significant need in synthetic biology and offering a versatile new tool for constructing programmable nucleic acids components in medicine, nanotechnology, and biocomputing.

TL;DR: This single-molecule methodology provides a sensitive readout of cocaine-binding based on the dissociation kinetics of the split aptamer, allowing one to distinguish target-dependent aptamer assembly from background assembly, and could be used to study other systems where target association affects the stability of aptamer duplexes.

TL;DR: EndoVIPER-seq (Endonuclease V inosine precipitation enrichment sequencing) is envisioned as a straightforward and cost-effective strategy to improve the epitranscriptomic informational density of RNA samples, facilitating a deeper understanding of the functional roles of A-to-I editing.

TL;DR: This is the first example of capture beads having more than one capture sequence, and it is envisioned that this technology will be of high utility for applications such as pairing the antigen receptor chains that give rise to autoimmune diseases or measuring the ratios of mRNA splice variants in cancer stem cells.

TL;DR: An acrylamidofluorescein reagent is reported that reacts with inosine and enables enrichment of inOSine-containing RNA transcripts and provides improved sensitivity in the detection and identification of inosines toward a more comprehensive transcriptome-wide analysis of A-to-I editing.