Jenny Bain

TL;DR: Harmine has been identified as a potent and specific inhibitor of DYRK1A (dual-specificity tyrosine-phosphorylated and -regulated kinase 1A) in vitro and the results have further emphasized the need for considerable caution in using small-molecule inhibitors of protein kinases to assess the physiological roles of these enzymes.

TL;DR: It is reported that there is a significant basal activity and phosphorylation of AMPK in LKB1-deficient cells that can be stimulated by Ca2+ ionophores, and studies using the CaMKK inhibitor STO-609 and isoform-specific siRNAs show thatCaMKKbeta is required for this effect.

TL;DR: The results suggest that the combined use of Roscovitine and Kenpaullone may be useful for identifying substrates and physiological roles of cyclin-dependent protein kinases, whereas the combineduse of Kenp Paullone and LiCl may be Useful for identifying substrateates and physiology roles of glycogen synthase kinase 3.

TL;DR: NDRG1 and NDRG2 are identified as physiological substrates for SGK1, and it is demonstrated that phosphorylation of N DRG1 by SGK 1 primes it for phosphorylated by GSK3.

TL;DR: The compound BIRB796 also inhibits the activity and activation of SAPK3/p38γ as discussed by the authors, which is a physiological substrate of p38α and p38β.