J
Jens Harborth
Researcher at Max Planck Society
Publications - 20
Citations - 14442
Jens Harborth is an academic researcher from Max Planck Society. The author has contributed to research in topics: RNA interference & RNA silencing. The author has an hindex of 15, co-authored 20 publications receiving 14091 citations.
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Journal ArticleDOI
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
TL;DR: 21-nucleotide siRNA duplexes provide a new tool for studying gene function in mammalian cells and may eventually be used as gene-specific therapeutics.
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Analysis of gene function in somatic mammalian cells using small interfering RNAs
TL;DR: Because of the robustness of the siRNA knockdown technology, genomewide analysis of human gene function in cultured cells has now become possible and here a collection of protocols for siRNA-mediated knockdown of mammalian gene expression is provided.
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Identification of essential genes in cultured mammalian cells using small interfering RNAs.
TL;DR: The first RNAi-induced phenotypes in mammalian cultured cells using RNA interference mediated by duplexes of 21-nt RNAs are reported, and two other lamins, B1 and B2, are now identified as essential proteins.
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Sequence, chemical, and structural variation of small interfering RNAs and short hairpin RNAs and the effect on mammalian gene silencing.
Jens Harborth,Sayda Elbashir,Kim Vandenburgh,Heiko Manninga,Stephen A. Scaringe,Klaus Weber,Thomas Tuschl +6 more
TL;DR: RNase-protecting phosphorothioate and 2'-fluoropyrimidine RNA backbone modifications of siRNAs did not significantly affect silencing efficiency, although cytotoxic effects were observed when every second phosphate of an siRNA duplex was replaced by phosphorOTHioate.
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Effect of Clathrin Heavy Chain- and α-Adaptin-specific Small Inhibitory RNAs on Endocytic Accessory Proteins and Receptor Trafficking in HeLa Cells
TL;DR: It is concluded that neither clathrin nor AP-2 is essential for the internalization of EGF, and it is taken up by an alternative mechanism.