Jimmy A. Guo

TL;DR: VIVO provides a general strategy for defining and quantifying the off-target effects of gene-editing nucleases in whole organisms, thereby providing a blueprint to foster the development of therapeutic strategies that use in vivo gene editing.

TL;DR: The results suggest that the CRISPR/Cas9 system used in this study is highly efficient and specific for genome editing in maize, while CRISpr/Cas12a needs further optimization for improved editing efficiency.

TL;DR: Overall, regenerative cell therapy based on SVF is at an early investigative stage but its potential for clinical application is enormous.

TL;DR: The existing literature suggests that angiogenesis, immunomodulation, differentiation, and extracellular matrix secretion are the main avenues through which regeneration and healing is achieved by the stromal vascular fraction.

TL;DR: The primary advantages of CIRCLE-seq as compared with other in vitro methods for defining genome-wide genome editing activity are (i) high enrichment for sequencing nuclease-cleaved gDNA/low background, enabling sensitive detection with low sequencing depth requirements; and (ii) the fact that paired-end reads can contain complete information on individual nuc lease cleavage sites, enabling use of C IRCLE- sequencing in species without high-quality reference genomes.