Showing papers by "Jin Hyung Lee published in 2017"
TL;DR: In this article, a dynamic causal modeling (DCM) for optogenetic fMRI experiments is proposed, which allows cell-type-specific, brain-wide functional measurements to parameterize the causal relationships among regions of a distributed brain network with cell type specificity.
60 citations
TL;DR: How targeting different brain regions and cell populations has opened up the possibility of highly specific therapies that can stop seizures on demand is illustrated and how combining newly available neuroscience tools with whole‐brain imaging techniques will allow researchers to understand better the spread of seizures on a network level.
Abstract: Currently, approximately 30% of patients with epilepsy do not have adequate seizure control. A greater understanding of the underlying mechanisms by which seizures start or propagate could lead to new therapeutic strategies. The recent development of optogenetics, because of its unprecedented precision for controlling activity within distinct neuronal populations, has revolutionized neuroscience, including epilepsy research. This Review discusses recent breakthroughs made with optogenetics in epilepsy research. These breakthroughs include new insights into the key roles that different cell types play in mediating seizures as well as in the development of epilepsy. Subsequently, we discuss how targeting different brain regions and cell populations has opened up the possibility of highly specific therapies that can stop seizures on demand. Finally, we illustrate how combining newly available neuroscience tools with whole-brain imaging techniques will allow researchers to understand better the spread of seizures on a network level. © 2016 The Authors. Journal of Neuroscience Research Published by Wiley Periodicals, Inc.
19 citations
TL;DR: The generation of a human embryonic stem cell (hESC) line with a tyrosine hydroxylase (TH)-RFP (red fluorescent protein) reporter is reported, validated that RFP faithfully mimicked TH expression during differentiation and enabled purification of mDA-like neurons from heterogeneous cultures.
17 citations
TL;DR: Key technologies that enable measurement and manipulation of neural activity and neural circuits are described, which led to the discovery of circuit mechanisms underlying pathological motor behavior, arousal regulation, and protein accumulation.
Abstract: Neurological disease drives symptoms through pathological changes to circuit functions. Therefore, understanding circuit mechanisms that drive behavioral dysfunction is of critical importance for quantitative diagnosis and systematic treatment of neurological disease. Here, we describe key technologies that enable measurement and manipulation of neural activity and neural circuits. Applying these approaches led to the discovery of circuit mechanisms underlying pathological motor behavior, arousal regulation, and protein accumulation. Finally, we discuss how optogenetic functional magnetic resonance imaging reveals global scale circuit mechanisms, and how circuit manipulations could lead to new treatments of neurological diseases.
17 citations
TL;DR: Analyses show that, in the presence of heterogeneous BOLD responses, conventionally used GLM with a canonical basis set leads to considerable errors in the detection and characterization of BOLDResponses.
15 citations
Patent•
30 Jan 2017TL;DR: In this article, methods and systems for analyzing brain functional activity data are provided. And systems that find use in performing the present methods are systems that can be used in performing these methods.
Abstract: Methods and systems for analyzing brain functional activity data are provided. Also provided are systems that find use in performing the present methods.
6 citations
Patent•
15 Jun 2017TL;DR: In this paper, the authors used optogenetics to stimulate a first region of a brain of an individual, in conjunction with functional magnetic resonance imaging (fMRI) of different regions of the brain to determine a dynamic functional connection between individual neurons of the first region and a second region.
Abstract: Provided herein are methods for analyzing in vivo a brain circuit. A method of the present disclosure may include using optogenetics to stimulate a first region of a brain of an individual, in conjunction with functional magnetic resonance imaging (fMRI) of different regions of the brain to determine a dynamic functional connection between individual neurons of the first region and a second region of the brain. The method may further include identifying a third region of the brain, the neurons of which region mediate the dynamic functional connection between the first and second regions.
1 citations