J
John Jeff Alvarado
Researcher at University of Pittsburgh
Publications - 16
Citations - 245
John Jeff Alvarado is an academic researcher from University of Pittsburgh. The author has contributed to research in topics: Proto-oncogene tyrosine-protein kinase Src & SH3 domain. The author has an hindex of 7, co-authored 11 publications receiving 192 citations. Previous affiliations of John Jeff Alvarado include Albert Einstein College of Medicine.
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Journal ArticleDOI
Effector Kinase Coupling Enables High-Throughput Screens for Direct HIV-1 Nef Antagonists with Antiretroviral Activity
Lori A. Emert-Sedlak,Purushottam Narute,Sherry T. Shu,Jerrod A. Poe,Haibin Shi,Naveena Yanamala,John Jeff Alvarado,John S. Lazo,John S. Lazo,Joanne I. Yeh,Paul A. Johnston,Thomas E. Smithgall +11 more
TL;DR: An assay that couples Nef to the activation of Hck, a Src family member and Nef effector protein is developed and a diphenylpyrazolo compound demonstrated submicromolar potency in HIV-1 replication assays against a broad range of primary Nef variants.
Journal ArticleDOI
Interaction with the Src Homology (SH3-SH2) Region of the Src-family Kinase Hck Structures the HIV-1 Nef Dimer for Kinase Activation and Effector Recruitment
TL;DR: The results suggest that in addition to serving as a kinase effector for Nef, Hck binding may reorganize the Nef dimer for functional interaction with other signaling partners.
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Structure of a microsporidian methionine aminopeptidase type 2 complexed with fumagillin and TNP-470.
John Jeff Alvarado,A. Nemkal,J.M. Sauder,M. Russell,Donna E. Akiyoshi,Wuxian Shi,Steven C. Almo,Louis M. Weiss +7 more
TL;DR: Comparison of microsporidian Met AP2 structures with human MetAP2 provides insights into the design of inhibitors that might exhibit specificity for microsporaidiosis, particularly in patients that are immunosuppressed.
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Crystal Structure of the Src Family Kinase Hck SH3-SH2 Linker Regulatory Region Supports an SH3-dominant Activation Mechanism.
TL;DR: The crystal structure of a truncated Hck protein consisting of the SH2 and SH3 domains plus the linker supports the idea that these noncatalytic regions work together as a “conformational switch” that modulates kinase activity in a manner unique to the SH3 domain and linker topologies present in the intact Hckprotein.
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Discovery of a diaminoquinoxaline benzenesulfonamide antagonist of HIV-1 Nef function using a yeast-based phenotypic screen
Ronald P. Trible,Purushottam Narute,Lori A. Emert-Sedlak,John Jeff Alvarado,Katelyn M. Atkins,Laurel Thomas,Toshiaki Kodama,Naveena Yanamala,Vasiliy N. Korotchenko,Billy W. Day,Gary Thomas,Thomas E. Smithgall +11 more
TL;DR: Inhibitors of Nef function discovered with this assay, such as DQBS, may complement the activity of current antiretroviral therapies by enabling immune recognition of HIV-infected cells through the rescue of cell surface MHC-I.