P
Purushottam Narute
Researcher at University of Pittsburgh
Publications - 5
Citations - 159
Purushottam Narute is an academic researcher from University of Pittsburgh. The author has contributed to research in topics: Proto-oncogene tyrosine-protein kinase Src & Kinase. The author has an hindex of 5, co-authored 5 publications receiving 144 citations.
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Journal ArticleDOI
Effector Kinase Coupling Enables High-Throughput Screens for Direct HIV-1 Nef Antagonists with Antiretroviral Activity
Lori A. Emert-Sedlak,Purushottam Narute,Sherry T. Shu,Jerrod A. Poe,Haibin Shi,Naveena Yanamala,John Jeff Alvarado,John S. Lazo,John S. Lazo,Joanne I. Yeh,Paul A. Johnston,Thomas E. Smithgall +11 more
TL;DR: An assay that couples Nef to the activation of Hck, a Src family member and Nef effector protein is developed and a diphenylpyrazolo compound demonstrated submicromolar potency in HIV-1 replication assays against a broad range of primary Nef variants.
Journal ArticleDOI
On the Solution Conformation and Dynamics of the HIV-1 Viral Infectivity Factor
Sean R. Marcsisin,Purushottam Narute,Lori A. Emert-Sedlak,Marek Kloczewiak,Thomas E. Smithgall,John R. Engen +5 more
TL;DR: Hydrogen exchange mass spectrometry (HX MS) was used to probe the solution conformation of recombinant full-length HIV-1 Vif, suggesting that the Vif studied by HX MS retained a biochemically competent conformation relevant to Hck interaction.
Journal ArticleDOI
Nef alleles from all major HIV-1 clades activate Src-family kinases and enhance HIV-1 replication in an inhibitor-sensitive manner.
TL;DR: It is demonstrated that the activation of Hck, Lyn and c-Src by Nef is highly conserved among all major clades of HIV-1 and that selective targeting of this pathway uniformly inhibits HIV- 1 replication.
Journal ArticleDOI
Discovery of a diaminoquinoxaline benzenesulfonamide antagonist of HIV-1 Nef function using a yeast-based phenotypic screen
Ronald P. Trible,Purushottam Narute,Lori A. Emert-Sedlak,John Jeff Alvarado,Katelyn M. Atkins,Laurel Thomas,Toshiaki Kodama,Naveena Yanamala,Vasiliy N. Korotchenko,Billy W. Day,Gary Thomas,Thomas E. Smithgall +11 more
TL;DR: Inhibitors of Nef function discovered with this assay, such as DQBS, may complement the activity of current antiretroviral therapies by enabling immune recognition of HIV-infected cells through the rescue of cell surface MHC-I.
Journal ArticleDOI
HIV-1 Nef interaction influences the ATP-binding site of the Src-family kinase, Hck
Teodora Pene-Dumitrescu,Sherry T. Shu,Thomas E. Wales,John Jeff Alvarado,Haibin Shi,Purushottam Narute,Jamie A. Moroco,Joanne I. Yeh,John R. Engen,Thomas E. Smithgall +9 more
TL;DR: Observations suggest that stable interaction with Nef may induce Src-family kinase active site conformations amenable to selective inhibitor targeting.