Showing papers by "John P. Iredale published in 1996"
TL;DR: Increased expression of tissue inhibitors of metalloproteinases relative to interstitial collagenase may promote deposition of interstitial collagens in liver fibrosis, and studies further suggest that lipocytes are an important source of tissue inhibitor of meetallop Proteinase 1 and 2 in progressive Liver fibrosis.
350 citations
TL;DR: It is suggested that increased expression of TIMP‐1 relative to interstitial collagenase by HSCs may promote progression of liver fibrosis in these rat models by preventing degradation of secreted collagens.
338 citations
Journal Article•
33 citations
TL;DR: Granulosa cells were prepared from follicular aspirates obtained at oocyte collection for in-vitro fertilization (IVF) and maintained in culture. as discussed by the authors showed that substantial loss of cells from the culture surface occurred in the absence of gonadotrophin when cells were maintained on a thin layer of extracellular matrix (ECM) using a defined, serum free medium.
Abstract: Granulosa cells were prepared from follicular aspirates obtained at oocyte collection for in-vitro fertilization (IVF) and maintained in culture. Substantial loss of cells from the culture surface occurred in the absence of gonadotrophin when cells were maintained on a thin layer of extracellular matrix (ECM) using a defined, serum-free medium. This cell loss was clearly and significantly reduced in the presence of human chorionic gonadotrophin (HCG) by days 4-6 of culture, and occurred in conjunction with loss of ECM. Analysis of culture medium by zymography using gelatin as substrate demonstrated the presence of metalloproteinases (MMP), MMP-9 (gelatinase B) appearing as the predominant band. Measurement of overall gelatinase activity in culture media revealed a progressive fall in gelatinase expressed on a per cell basis in media from HCG-treated cultures and this was less marked in controls. This suppression of gelatinase activity was consistent with an observed increase in production of tissue inhibitor of metalloproteinase-1 (TIMP-1) by HCG-treated cells, which was significant by days 6-8 of culture. We speculate that stabilization of the ECM may be an important aspect of HCG action in the corpus luteum.
27 citations
TL;DR: Although bezoars are rare, it is important that the clinician is aware of the possibility of their development, particularly in susceptible individuals, and recourse to laparotomy is frequently required to treat them.
Abstract: A wide variety of drugs or combinations of drugs have the potential to form bezoars. In the majority of patients presenting with bezoars there is a clear predisposing factor. This article highlights those drugs or groups of drugs which have been implicated in bezoar formation. Although bezoars are rare, it is important that the clinician is aware of the possibility of their development, particularly in susceptible individuals. This is because bezoars often develop in the elderly and in those with serious coexistent disease. They may be difficult to diagnose and recourse to laparotomy is frequently required to treat them. As a result, they are associated with a significant morbidity and mortality.
8 citations