Jonathan Widom

TL;DR: This work isolated nucleosome-bound sequences at high resolution from yeast and used these sequences in a new computational approach to construct and validate experimentally a nucleosom–DNA interaction model, and to predict the genome-wide organization of nucleosomes.

TL;DR: The results indicate that the intrinsic DNA sequence preferences of nucleosomes have a central role in determining the organization ofucleosomes in vivo.

TL;DR: These results explain how remodeling factors can be recruited to particular nucleosomes on a biologically relevant timescale, and imply that the major impediment to entry of RNA polymerase into a nucleosome is rewrapping of nucleosomal DNA, not unwrapping.

TL;DR: A genome-wide map of ∼380,000 yeast nucleosomes is used and it is found that Poly(dA:dT) tracts are an important component of these nucleosome positioning signals and that their nucleosom-disfavoring action results in large nucleosite depletion over them and over their flanking regions and enhances the accessibility of transcription factors to their cognate sites.

TL;DR: It is proposed that the Hat2p/Rbap48 family serve as escorts of histone metabolism enzymes to facilitate their interaction with histone H4.