Yvonne N. Fondufe-Mittendorf

TL;DR: This work isolated nucleosome-bound sequences at high resolution from yeast and used these sequences in a new computational approach to construct and validate experimentally a nucleosom–DNA interaction model, and to predict the genome-wide organization of nucleosomes.

TL;DR: The results indicate that the intrinsic DNA sequence preferences of nucleosomes have a central role in determining the organization ofucleosomes in vivo.

TL;DR: A genome-wide map of ∼380,000 yeast nucleosomes is used and it is found that Poly(dA:dT) tracts are an important component of these nucleosome positioning signals and that their nucleosom-disfavoring action results in large nucleosite depletion over them and over their flanking regions and enhances the accessibility of transcription factors to their cognate sites.

TL;DR: It is found that recruitment of the yeast INO1 and GAL1 genes to the nuclear periphery is rapid and independent of transcription, which represents an epigenetic state that confers memory of transcriptional activation and promotes reactivation.

TL;DR: A large fraction of nucleosomes are consistently positioned—in some regions because they adopt favored sequence positions, and in other regions becausethey are forced into specific arrangements by chromatin remodeling or DNA binding proteins.