Bioaerosols in the Earth system: Climate, health, and ecosystem interactions

https://www.bmj.com/content/bmj/1/4910/399.2.full.pdf

Physiology and biochemistry of the skin

Numerical simulations of asteroid breakups, including both the fragmentation of the parent body and the gravitational interactions between the fragments, have allowed us to reproduce successfully the main properties of asteroid families formed in different regimes of impact energy, starting from a non-porous parent body. In this paper, using the same approach, we concentrate on a single regime of impact energy, the so-called catastrophic threshold usually designated by Q D * , which results in the escape of half of the target’s mass. Thanks to our recent implementation of a model of fragmentation of porous materials, we can characterize Q D * for both porous and non-porous targets with a wide range of diameters. We can then analyze the potential influence of porosity on the value of Q D * , and by computing the gravitational phase of the collision in the gravity regime, we can characterize the collisional outcome in terms of the fragment size and ejection speed distributions, which are the main outcome properties used by collisional models to study the evolutions of the different populations of small bodies. We also check the dependency of Q D * on the impact speed of the projectile. In the strength regime, which corresponds to target sizes below a few hundreds of meters, we find that porous targets are more difficult to disrupt than non-porous ones. In the gravity regime, the outcome is controlled purely by gravity and porosity in the case of porous targets. In the case of non-porous targets, the outcome also depends on strength. Indeed, decreasing the strength of non-porous targets make them easier to disrupt in this regime, while increasing the strength of porous targets has much less influence on the value of Q D * . Therefore, one cannot say that non-porous targets are systematically easier or more difficult to disrupt than porous ones, as the outcome highly depends on the assumed strength values. In the gravity regime, we also confirm that the process of gravitational reaccumulation is at the origin of the largest remnant’s mass in both cases. We then propose some power-law relationships between Q D * and both target’s size and impact speed that can be used in collisional evolution models. The resulting fragment size distributions can also be reasonably fitted by a power-law whose exponent ranges between −2.2 and −2.7 for all target diameters in both cases and independently on the impact velocity (at least in the small range investigated between 3 and 5 km/s). Then, although ejection velocities in the gravity regime tend to be higher from porous targets, they remain on the same order as the ones from non-porous targets.

Fragment Properties at the Catastrophic Disruption Threshold: The Effect of the Parent Body's Internal Structure

Author(s): Bhangar, S; Huffman, JA; Nazaroff, WW | Abstract: UnlabelledThis study is among the first to apply laser-induced fluorescence to characterize bioaerosols at high time and size resolution in an occupied, common-use indoor environment. Using an ultraviolet aerodynamic particle sizer, we characterized total and fluorescent biological aerosol particle (FBAP) levels (1-15 μm diameter) in a classroom, sampling with 5-min resolution continuously during eighteen occupied and eight unoccupied days distributed throughout a one-year period. A material-balance model was applied to quantify per-person FBAP emission rates as a function of particle size. Day-to-day and seasonal changes in FBAP number concentration (NF ) values in the classroom were small compared to the variability within a day that was attributable to variable levels of occupancy, occupant activities, and the operational state of the ventilation system. Occupancy conditions characteristic of lecture classes were associated with mean NF source strengths of 2 × 10(6) particles/h/person, and 9 × 10(4) particles per metabolic g CO2 . During transitions between lectures, occupant activity was more vigorous, and estimated mean, per-person NF emissions were 0.8 × 10(6) particles per transition. The observed classroom peak in FBAP size at 3-4 μm is similar to the peak in fluorescent and biological aerosols reported from several studies outdoors.Practical implicationsCoarse particles that exhibit fluorescence at characteristic wavelengths are considered to be proxies for biological particles. Recently developed instruments permit their detection and sizing in real time. In a mechanically ventilated classroom, emissions from human occupants were a strong determinant of coarse-mode fluorescent biological aerosol particle (FBAP) levels. Human FBAP emission rates were significant under quiet occupancy conditions and increased with activity level. Fluorescent particle emissions peaked at a diameter of 3–4 μm, which is the expected modal size of airborne particles with associated microbes. Human activity patterns, and associated coarse FBAP and total particle levels varied strongly on short timescales. Thus, the dynamic temporal behavior of aerosol concentrations must be considered when determining collection protocols for samples meant to be representative of average concentrations using time-integrated or ‘snapshot’ bioaerosol measurement techniques.

https://onlinelibrary.wiley.com/doi/pdf/10.1111/ina.12111

Size-resolved fluorescent biological aerosol particle concentrations and occupant emissions in a university classroom.

A review of mechanisms and models for dynamic failure, strength, and fragmentation

The purpose of this review is to investigate the feasibility of bioaerosol fingerprinting based on current understanding of cellular debris (with emphasis on human-emitted particulates) in aerosols and arguments regarding sampling, sensitivity, separations, and detection schemes. Target aerosol particles include cellular material and proteins emitted by humans, animals, and plants and can be regarded as information-rich packets that carry biochemical information specific to the living organisms present where the sample is collected. In this work we discuss sampling and analysis techniques that can be integrated with molecular (e.g. protein)-detection procedures to properly assess the aerosolized cellular material of interest. Developing a detailed understanding of bioaerosol molecular profiles in different environments suggests exciting possibilities of bioaerosol analysis with applications ranging from military defense to medical diagnosis and wildlife identification

Exploring the feasibility of bioaerosol analysis as a novel fingerprinting technique.

https://digitalcommons.newhaven.edu/cgi/viewcontent.cgi?article=1030&context=forensicscience-facpubs

Morphologically-directed Raman spectroscopy for forensic soil analysis

Automated microscopy and particle size analysis of dynamic fragmentation in natural ceramics

Spontaneous formation of long-range (millimeters) membrane-bound nanotubules from surface-immobilized liposomes is possible by application of modest electric fields (2 -20 V/cm), providing a novel fabrication strategy for these hollow cylindrical structures. Stable tubes generally aligned with the applied electric field were created from liposomes prepared with phosphatidylcholine (PC), phosphatidic acid (PA), phosphoethanolamine (PE), and cholesterol. The minimum voltage which causes nanotubular formation (the onset voltage) and the average number of tubules per liposome of varying composition was examined with fluorescent microscopy using labeled phospholipids. Generally, the onset voltages ranged between 4 and 15 V/cm and depended on the mother vesicle composition. The results of this study suggest that increasing the charged lipid content can decrease the onset voltage. Conversely, a cholesterol content of more than 30% (by mass) was found to hinder extension of lipid tubules. Basic calculations that assume lipid migration and domain formation on the mother liposome as a nucleating site for tubule extension are assessed and suggest this is a reasonable model to describe the mechanism of tubular growth from immobilized liposomes.

/pdf/bionanotubule-formation-from-surface-attached-liposomes-4slc02xfwk.pdf

Bionanotubule formation from surface-attached liposomes using electric fields.

https://www.public.asu.edu/%7Emhayes/publications/2014/Staton_Ecological%20Indicators_2014.pdf

Josemar A. Castillo

Papers

Exploring the feasibility of bioaerosol analysis as a novel fingerprinting technique.

Morphologically-directed Raman spectroscopy for forensic soil analysis

Automated microscopy and particle size analysis of dynamic fragmentation in natural ceramics

Bionanotubule formation from surface-attached liposomes using electric fields.

Ground level environmental protein concentrations in various ecuadorian environments: Potential uses of aerosolized protein for ecological research