J
Joshua A. Weinstein
Researcher at Broad Institute
Publications - 18
Citations - 8960
Joshua A. Weinstein is an academic researcher from Broad Institute. The author has contributed to research in topics: Antibody Repertoire & Immune receptor. The author has an hindex of 15, co-authored 17 publications receiving 7861 citations. Previous affiliations of Joshua A. Weinstein include Massachusetts Institute of Technology & University of Pennsylvania.
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Journal ArticleDOI
DNA targeting specificity of RNA-guided Cas9 nucleases
Patrick D. Hsu,David A. Scott,David A. Scott,Joshua A. Weinstein,Joshua A. Weinstein,F. Ann Ran,F. Ann Ran,F. Ann Ran,Silvana Konermann,Silvana Konermann,Vineeta Agarwala,Vineeta Agarwala,Vineeta Agarwala,Yinqing Li,Yinqing Li,Eli J. Fine,Xuebing Wu,Ophir Shalem,Ophir Shalem,Thomas J. Cradick,Luciano A. Marraffini,Gang Bao,Feng Zhang,Feng Zhang +23 more
TL;DR: In this article, the Streptococcus pyogenes Cas9 (SpCas9) nuclease can be efficiently targeted to genomic loci by means of single-guide RNAs (sgRNAs) to enable genome editing.
DNA targeting specificity of RNA-guided Cas9 nucleases
Patrick D. Hsu,David A. Scott,David A. Scott,Joshua A. Weinstein,Joshua A. Weinstein,F. Ann Ran,F. Ann Ran,F. Ann Ran,Silvana Konermann,Silvana Konermann,Vineeta Agarwala,Vineeta Agarwala,Vineeta Agarwala,Yinqing Li,Yinqing Li,Eli J. Fine,Xuebing Wu,Ophir Shalem,Ophir Shalem,Thomas J. Cradick,Luciano A. Marraffini,Gang Bao,Feng Zhang,Feng Zhang +23 more
TL;DR: It is found that SpCas9 tolerates mismatches between guide RNA and target DNA at different positions in a sequence-dependent manner, sensitive to the number, position and distribution of mismatches.
Journal ArticleDOI
High-Throughput Sequencing of the Zebrafish Antibody Repertoire
TL;DR: High-throughput sequencing of the variable domain of the antibody heavy chain from 14 zebrafish is used to provide insight into the breadth of the expressed antibody repertoire and immunological diversity at the level of an individual organism.
Journal ArticleDOI
Lineage Structure of the Human Antibody Repertoire in Response to Influenza Vaccination
Ning Jiang,Jiankui He,Joshua A. Weinstein,Lolita Penland,Sanae Sasaki,Sanae Sasaki,Xiao-Song He,Xiao-Song He,Cornelia L. Dekker,Nai Ying Zheng,Min Huang,Meghan Sullivan,Patrick C. Wilson,Harry B. Greenberg,Harry B. Greenberg,Mark M. Davis,Mark M. Davis,Daniel S. Fisher,Stephen R. Quake +18 more
TL;DR: Global analysis of the immune system’s clonal structure provides direct insight into the effects of vaccination and provides a detailed molecular portrait of age-related effects, using high-throughput long read sequencing to characterize the human antibody response after influenza vaccination.
Journal ArticleDOI
Genetic measurement of memory B-cell recall using antibody repertoire sequencing.
TL;DR: A consensus read sequencing approach that incorporates unique barcode labels on each starting RNA molecule enables accurate quantification of RNA and isotype levels and validated this approach and analyzed the differential response of the antibody repertoire to live-attenuated or trivalent-inactivated influenza vaccination.