E
Eli J. Fine
Researcher at Georgia Institute of Technology
Publications - 19
Citations - 9422
Eli J. Fine is an academic researcher from Georgia Institute of Technology. The author has contributed to research in topics: Genome editing & Transcription activator-like effector nuclease. The author has an hindex of 15, co-authored 19 publications receiving 8224 citations. Previous affiliations of Eli J. Fine include Georgia Tech Research Institute & Stanford University.
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Journal ArticleDOI
DNA targeting specificity of RNA-guided Cas9 nucleases
Patrick D. Hsu,David A. Scott,David A. Scott,Joshua A. Weinstein,Joshua A. Weinstein,F. Ann Ran,F. Ann Ran,F. Ann Ran,Silvana Konermann,Silvana Konermann,Vineeta Agarwala,Vineeta Agarwala,Vineeta Agarwala,Yinqing Li,Yinqing Li,Eli J. Fine,Xuebing Wu,Ophir Shalem,Ophir Shalem,Thomas J. Cradick,Luciano A. Marraffini,Gang Bao,Feng Zhang,Feng Zhang +23 more
TL;DR: In this article, the Streptococcus pyogenes Cas9 (SpCas9) nuclease can be efficiently targeted to genomic loci by means of single-guide RNAs (sgRNAs) to enable genome editing.
DNA targeting specificity of RNA-guided Cas9 nucleases
Patrick D. Hsu,David A. Scott,David A. Scott,Joshua A. Weinstein,Joshua A. Weinstein,F. Ann Ran,F. Ann Ran,F. Ann Ran,Silvana Konermann,Silvana Konermann,Vineeta Agarwala,Vineeta Agarwala,Vineeta Agarwala,Yinqing Li,Yinqing Li,Eli J. Fine,Xuebing Wu,Ophir Shalem,Ophir Shalem,Thomas J. Cradick,Luciano A. Marraffini,Gang Bao,Feng Zhang,Feng Zhang +23 more
TL;DR: It is found that SpCas9 tolerates mismatches between guide RNA and target DNA at different positions in a sequence-dependent manner, sensitive to the number, position and distribution of mismatches.
Journal ArticleDOI
CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity
TL;DR: It is demonstrated that CRISPR/Cas9 systems targeting the human hemoglobin β and C-C chemokine receptor type 5 genes have substantial off-target cleavage, especially within the hemoglobin δ and C of 2 genes, respectively, causing gross chromosomal deletions.
Journal ArticleDOI
COSMID: A Web-based Tool for Identifying and Validating CRISPR/Cas Off-target Sites
TL;DR: A bioinformatics-based tool, COSMID (CRISPR Off-target Sites with Mismatches, Insertions, and Deletions) that searches genomes for potential off-target sites, thus helping the design of CRISPR/Cas systems with minimal off- target effects, as well as the identification and quantification of CRispr/Cas induced off- Target cleavage in cells.
Journal ArticleDOI
TALENs facilitate targeted genome editing in human cells with high specificity and low cytotoxicity
Claudio Mussolino,Jamal Alzubi,Eli J. Fine,Robert Morbitzer,Thomas J. Cradick,Thomas Lahaye,Gang Bao,Toni Cathomen +7 more
TL;DR: The results link nuclease-associated toxicity to off-target cleavage activity and corroborate TALENs as a highly specific platform for future clinical translation.