Juan Codina

TL;DR: This chapter discusses the progress that has been made in the purification of various components of the adenylate cyclase system and presents various types of reconstitution approaches that have been used to monitor the interactions of the β -adrenergic receptor with the other components of this system.

TL;DR: The authors' interest in calcium channels was prompted by the results of studies published by Hescheler and collaborators, which showed that the inhibitory regulation of the voltage gate calcium channels in NG108-15 cells in response to opioids is reconstituted after pertussis toxin treatment with Go as well as Gi.

TL;DR: Data show that a primary alteration of the alpha s gene coding sequence is not responsible for defective Gs-associated signal transduction in dysautonomic MVP patients, and suggest that the molecular lesion could be an abnormal posttranslational modification of alpha s, a defect in the beta or gamma subunits of Gs, or an unusual interaction between the subunits in the Gs of these patients.

TL;DR: This chapter discusses the molecular basis of signal transduction by G proteins: the pros and cons of whether G proteins dissociate into α plus βγs in the normal phospholipid environment and, if so, what can be said about the role that this dissociation reaction plays in the mode of action of receptors; and the α vs. βγ controversy that has arisen in two areas of G protein action.