Juan Codina

TL;DR: It is concluded that the responses seen are due to Gs rather than a contaminant, that the effect on Ca2+ channel activity is that of a true stimulation, akin to that on adenylyl cyclase, and that a given G protein may regulate more than one effector system.

TL;DR: The established hormone responsive activity retains the beta 2-adrenergic specificity conferred by the pure receptor, and similar extents of stimulation are observed with pure receptor from frog erythrocytes, indicating a similar efficiency of coupling between receptors from different species and NS.

TL;DR: Brain Go can couple diverse brain potassium channels to neurotransmitter receptors, and four distinct types of potassium channels were activated by both isolated brain G0 and recombinant Go alpha.

TL;DR: This work believes that it has purified a guanine nucleotide- and Mg2+-binding inhibitory regulatory component of adenylyl cyclases--i.e., the Ni.

TL;DR: Methods were developed to adequately extract, separate and, without the use of NaF as stabilizing agent, purify to better than 90% purity human erythrocyte Ns and Ni, the stimulatory and inhibitory guanine nucleotide- and Mg-binding regulatory components of adenylyl cyclases, as well as a protein containing Mr = 35,000 subunits.