Juan Codina

TL;DR: The effect of GTP analogs and Mg on the structure of Ns and Ni, the stimulatory and inhibitory regulatory components of adenylyl cyclase, were studied in a comparative manner and a plausible description of the reaction sequence leading from an unactivated to an activated N protein is suggested.

TL;DR: Northern analysis with specifically designed oligonucleotides indicates that both forms of alpha o are expressed in normal tissues, e.g. brain, and proposes that alpha o1 and alpha o2 result as a consequence of alternative splicing of a single alpha o transcript.

TL;DR: This article immunized rabbits with purified guanine nucleotide-binding proteins (G proteins) from bovine brain and obtained an antiserum, RV3, that reacts specifically with the alpha subunit (39 kDa) of a G protein of unknown function, termed Go, as well as with the beta subunit common to all G proteins.

TL;DR: The results described in this report document the feasibility of studying hormone-responsive adenylate cyclase in a totally reconstituted system which retains the major regulatory properties of the enzyme in its native membrane-bound environment.

TL;DR: This work proves for the first time that the β‐subunits of all signal‐transducing G‐proteins, including transducin, are the same.