J
Jun Hiratake
Researcher at Kyoto University
Publications - 108
Citations - 3799
Jun Hiratake is an academic researcher from Kyoto University. The author has contributed to research in topics: Catalysis & Lipase. The author has an hindex of 32, co-authored 108 publications receiving 3493 citations.
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Journal ArticleDOI
Structural basis for the spectral difference in luciferase bioluminescence.
Toru Nakatsu,Susumu Ichiyama,Jun Hiratake,Adrian Saldanha,Nobuyuki Kobashi,Kanzo Sakata,Hiroaki Kato +6 more
TL;DR: The results indicate that the degree of molecular rigidity of the excited state of oxyluciferin, which is controlled by a transient movement of Ile 288, determines the colour of bioluminescence during the emission reaction.
Journal ArticleDOI
Sensitive β-galactosidase-targeting fluorescence probe for visualizing small peritoneal metastatic tumours in vivo
Daisuke Asanuma,Masayo Sakabe,Mako Kamiya,Kyoko Yamamoto,Jun Hiratake,Mikako Ogawa,Nobuyuki Kosaka,Peter L. Choyke,Tetsuo Nagano,Hisataka Kobayashi,Yasuteru Urano +10 more
TL;DR: The results clearly indicate preclinical potential value of the probe for fluorescence-guided diagnosis of peritoneal metastases from ovarian cancers and endoscopic fluorescence detection of metastases is demonstrated.
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Molecular Cloning and Characterization of Bifidobacterium bifidum 1,2-α-l-Fucosidase (AfcA), a Novel Inverting Glycosidase (Glycoside Hydrolase Family 95)
Takane Katayama,Akiko Sakuma,Kimura Takatoshi,Yutaka Makimura,Jun Hiratake,Kanzo Sakata,Takashi Yamanoi,Hidehiko Kumagai,Kenji Yamamoto +8 more
TL;DR: It was revealed that the AfcA protein constitutes a novel inverting GH family (GH family 95) and exhibited no similarity to those of any glycoside hydrolases (GHs) but showed high similarity to several hypothetical proteins in a database.
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Structural basis for gating mechanisms of a eukaryotic P-glycoprotein homolog
Atsushi Kodan,Tomohiro Yamaguchi,Toru Nakatsu,Keita Sakiyama,Christopher J. Hipolito,Akane Fujioka,Ryo Hirokane,Keiji Ikeguchi,Bunta Watanabe,Jun Hiratake,Yasuhisa Kimura,Hiroaki Suga,Kazumitsu Ueda,Hiroaki Kato +13 more
TL;DR: The high-resolution crystal structures of a eukaryotic P-glycoprotein homolog are determined and revealed the detailed architecture of its transmembrane domains, which contain an exit gate for substrates that opens to the extracellular side and two entrance gates that open to the intramembranous region and the cytosolic side.
Journal ArticleDOI
Identification and Molecular Cloning of a Novel Glycoside Hydrolase Family of Core 1 Type O-Glycan-specific Endo-α-N-acetylgalactosaminidase from Bifidobacterium longum
Kiyotaka Fujita,Fusako Oura,Noriko Nagamine,Takane Katayama,Jun Hiratake,Kanzo Sakata,Hidehiko Kumagai,Kenji Yamamoto +7 more
TL;DR: This is the first report for the isolation of a gene encoding endo-α-N-acetylgalactosaminidase from any organisms and for the establishment of a new glycoside hydrolase family (GH family 101).