J
Jun Lee
Publications - 2
Citations - 610
Jun Lee is an academic researcher. The author has contributed to research in topics: Complementary DNA & Gene. The author has an hindex of 2, co-authored 2 publications receiving 432 citations.
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Using single nuclei for RNA-seq to capture the transcriptome of postmortem neurons
Suguna Rani Krishnaswami,Rashel V. Grindberg,Mark Novotny,Pratap Venepally,Benjamin Lacar,Kunal Bhutani,Sara B. Linker,Son Pham,Jennifer A. Erwin,Jeremy A. Miller,Rebecca D. Hodge,James K. McCarthy,Martijn J. E. Kelder,Jamison McCorrison,Brian D. Aevermann,Francisco Diez Fuertes,Francisco Diez Fuertes,Richard H. Scheuermann,Jun Lee,Ed S. Lein,Nicholas J. Schork,Michael J. McConnell,Fred H. Gage,Roger S. Lasken +23 more
TL;DR: A protocol is described for sequencing the transcriptome of a cell nucleus by isolating nuclei at 4 °C from tissue homogenates, which cause minimal damage and allows investigation of biological features unique to nuclei, such as enrichment of certain transcripts and precursors of some noncoding RNAs.
Journal ArticleDOI
RNA-sequencing from single nuclei
Rashel V. Grindberg,Joyclyn Yee-Greenbaum,Michael J. McConnell,Mark Novotny,Andrew O'Shaughnessy,Georgina M. Lambert,Marcos J. Araúzo-Bravo,Jun Lee,Max Fishman,Gillian E. Robbins,Xiaoying Lin,Pratap Venepally,Jonathan H. Badger,David W. Galbraith,Fred H. Gage,Roger S. Lasken +15 more
TL;DR: In this paper, the authors demonstrate that this method can be done using the extremely low levels of mRNA in a single nucleus, isolated from a mouse neural progenitor cell line and from dissected hippocampal tissue.