Jun Y. Axup

TL;DR: Genetically encoded unnatural amino acids with orthogonal chemical reactivity are used to synthesize homogeneous ADCs with precise control of conjugation site and stoichiometry to facilitate the optimization of ADCs for a host of therapeutic uses.

TL;DR: The site-specific incorporation of unnatural amino acids with orthogonal chemical reactivity into proteins enables the synthesis of structurally defined protein conjugates, especially the next-generation protein therapeutics.

TL;DR: An anti-HER2/anti-CD3 bispecific antibody is synthesized, which efficiently cross-linked HER2+ cells and CD3+ cells, and in vitro effector-cell mediated cytotoxicity was observed at picomolar concentrations.

TL;DR: It is shown that unnatural amino acids with orthogonal chemical reactivity can be used to generate site-specific antibody-oligonucleotide conjugates that recruit cytotoxic T lymphocytes to Her2 and CD20 positive cancer cells and multimeric antibody fragments with enhanced activity.

TL;DR: The experiments demonstrate that the combined application of 2D IR spectroscopy and site-specific incorporation of VDLs can provide information on dynamics, structure, and interactions at virtually any site throughout any protein.