Isolation of lipase-secreting bacteria by deploying used frying oil as selective substrate - science tackles a potential health hazard

Municipal solid waste management: Dynamics, risk assessment, ecological influence, advancements, constraints and perspectives.

Valorization of agro-industrial wastes for biorefinery process and circular bioeconomy: A critical review.

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Valorization of agro-industrial wastes for biorefinery process and circular bioeconomy: A critical review

Production of biosurfactants from agro-industrial waste and waste cooking oil in a circular bioeconomy: An overview.

Actinomycete strains from cottonseed soap-stock (a dark gelatinous waste from oil refinery) were obtained. Cultures were analyzed for the enzyme lipase, protease and cellulase production using plate assay(s). Best lipolytic isolates were further quantitatively analyzed for lipase production, using substrate p-nitrophenyl palmitate (p-NPP). Cultures were characterized by morphological and molecular studies. The potent lipase producing strain of actinomycete was optimized for pH, temperature, inoculum size, carbon and nitrogen sources. Further, the lipolytic strain of actinomycete was grown on synthetic media and growth factors were optimized by RSM (Response Surface Methodology) for maximum production of lipase. Total 49 cultures were obtained from enriched soap-stock samples; among them 7 isolates were strains of actinomycete. Potent actinomycete strains were identified as Nocardiopsis alba, Streptomyces leeuwenhoekii, Streptomyces caelestis, Streptomyces werraensis and Streptomyces sclerotialus. Based on quantitative lipase production study, Nocardiopsis alba was selected as the best lipase producer. Optimized results of process parameter studies for lipase production by Nocardiopsis alba were: 5mL inoculum size, temperature 40 ∘ C, shaking at 130 rpm, pH 8.0 and cottonseed oil as the best carbon source. Cottonseed oil, K2HPO4 and cellulose were noted as main factors as optimized by Central Composite Design (CCD), where the culture showed highest (65.78 U/mL/min) lipase production. Findings of the research would offer potential environmental benefits along with socio-economic benefits if implemented suitably.

Process parameter studies by central composite design of response surface methodology for lipase activity of newly obtained Actinomycete

Background/Objectives: The present study was focused to exploit the indigenous strains of fungi isolated from cotton seed soapstock for the production of the extracellular lipase through submerged fermentation technique. Methods/ Statistical analysis: Cotton seed soapstock samples used in the study contains gelatinous oil richer chemical constituents. In addition, their enrichment and diluted materials were used for the isolation of lipase producing microorganisms on tributyrin agar plates. All isolates were lipase positive confirmed by a qualitative plate assay. Quantitative estimation of Lipase production activity was measured spectrophotometrically using pnitrophenyl palmitate (p-NPP) as a substrate. In order to exploit the isolated fungal strain for industrial usage, other cellulase and protease enzymes were tested by plate assay. Morphological and molecular characteristics of selected isolates were studied. Findings: From enriched soapstock samples, a total of 49 cultures were isolated among them 19 fungal isolates were screened for lipase, cellulase, and protease activity qualitatively by plate assay. Out of 19, six fungi were selected based on their lipase activity. Highly potent Fusarium solani 7F had the ability to produce 5.95 U/mL/min. crude lipase whereas Aspergillus niger 13F has 4.2 U/mL/min. after 4days incubation at 30oC. Potent fungi culture was identified by morphological, cultural, and molecular characteristics (18s rRNA gene sequence and phylogenetic analysis) revealed them as Penicillium griseofulvum 5F, Aspergillus flavus 6F, Fusarium solani 7F, Aspergillus niger 12F, Aspergillus niger 13F, and Aspergillus terreus 17F. Novelty: Fungi was the first time reported and isolated from cotton seed soapstock materials. In future studies, this enzyme will be used in the degradation of soapstock and also in the production of biosurfactant from soapstock.
Keywords: Cotton seed soapstock; Fusarium solani 7F; p-nitrophenyl palmitate; Tributyrin agar plates; degradation

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Kamlesh R. Shah

Papers

Process parameter studies by central composite design of response surface methodology for lipase activity of newly obtained Actinomycete

Isolation, screening and identification of Lipase producing fungi from cotton seed soapstock

Bioethanol Production From Pulp Of Fruits

Biodegradation of cotton seed soapstocks by novel indigenous Bacillus species