K
Kazuo Takayama
Researcher at Kyoto University
Publications - 128
Citations - 3501
Kazuo Takayama is an academic researcher from Kyoto University. The author has contributed to research in topics: Induced pluripotent stem cell & Medicine. The author has an hindex of 25, co-authored 103 publications receiving 2293 citations. Previous affiliations of Kazuo Takayama include Osaka University & National Presto Industries.
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Journal ArticleDOI
3D spheroid culture of hESC/hiPSC-derived hepatocyte-like cells for drug toxicity testing
Kazuo Takayama,Kenji Kawabata,Yasuhito Nagamoto,Keisuke Kishimoto,Katsuhisa Tashiro,Fuminori Sakurai,Masashi Tachibana,Katsuhiro Kanda,Takao Hayakawa,Miho Furue,Hiroyuki Mizuguchi +10 more
TL;DR: A hepatocyte differentiation method that employs not only stage-specific transient overexpression of hepatocyte-related transcription factors but also a three-dimensional spheroid culture system using a Nanopillar Plate is established that could generate more matured hepatocytes-like cells than the previous protocol.
Journal ArticleDOI
Efficient Generation of Functional Hepatocytes From Human Embryonic Stem Cells and Induced Pluripotent Stem Cells by HNF4α Transduction
Kazuo Takayama,Mitsuru Inamura,Kenji Kawabata,Kazufumi Katayama,Maiko Higuchi,Katsuhisa Tashiro,Aki Nonaka,Fuminori Sakurai,Takao Hayakawa,Takao Hayakawa,Miho Furue,Hiroyuki Mizuguchi +11 more
TL;DR: The method would be a valuable tool for the efficient generation of functional hepatocytes derived from human ESCs and iPSCs, and the hepatocyte-like cells could be used for predicting drug toxicity and could catalyze the toxication of several compounds.
Journal ArticleDOI
Prediction of interindividual differences in hepatic functions and drug sensitivity by using human iPS-derived hepatocytes
Kazuo Takayama,Yuta Morisaki,Shuichi Kuno,Yasuhito Nagamoto,Kazuo Harada,Norihisa Furukawa,Manami Ohtaka,Ken Nishimura,Kazuo Imagawa,Fuminori Sakurai,Masashi Tachibana,Ryo Sumazaki,Emiko Noguchi,Mahito Nakanishi,Kazumasa Hirata,Kenji Kawabata,Hiroyuki Mizuguchi +16 more
TL;DR: It is found that cytochrome P450 metabolism capacity and drug responsiveness of the primary human hepatocytes–human induced pluripotent stem cells–hepatocyte-like cells (PHH-iPS-HLCs) were highly correlated with those of PHHs, suggesting that the PHH- iPS- HLCs retained donor-specific CYP metabolism capacityand drug responsiveness.
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Generation of metabolically functioning hepatocytes from human pluripotent stem cells by FOXA2 and HNF1α transduction
Kazuo Takayama,Mitsuru Inamura,Kenji Kawabata,Michiko Sugawara,Kiyomi Kikuchi,Maiko Higuchi,Yasuhito Nagamoto,Hitoshi Watanabe,Katsuhisa Tashiro,Fuminori Sakurai,Takao Hayakawa,Takao Hayakawa,Miho Furue,Hiroyuki Mizuguchi +13 more
TL;DR: The method employing the transduction of FOXA2 and HNF1α represents a useful tool for the efficient generation of metabolically functional hepatocytes from hESCs and hiPSCs, and the screening of drug-induced cytotoxicity.
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In Vitro and Animal Models for SARS-CoV-2 research.
TL;DR: Here, it is briefly reviewed the cell lines, organoids, and animal models that are currently being used in COVID-19 research.