Keiichi Takeishi

TL;DR: It is the first clear demonstration that a codon containing adenosine residue in the third letter was specifically recognized by a tRNA.

TL;DR: Evidence is provided that yeast N-formylmethionyl-tRNA I serves as an initiator of protein synthesis programmed with the natural messenger in an E. coli system in vitro.

TL;DR: Yeast tRNA Glu 3, which recognizes GAA but not GAG as codon for glutamic acid, was purified by column chromatography to approx.

TL;DR: A yeast glutamic acid tRNA, which specifically recognizes GAA codon but not GAG codon, was digested with RNase T1 and the nucleotide sequences of the fragments were determined and a minor nucleoside, 2-thiouridine derivative, was found in the presumed anticodon.

TL;DR: The total nucleotide sequence of tRNAGlu3 from baker's yeast was determined and the existence of a minor nucleoside, 2-thiouridine-5-acetic acid methyl ester, was confirmed at the “Wobble position” in the anticodon of this tRNA.