Takao Sekiya

TL;DR: The mobility shift analysis of single-stranded DNAs on neutral polyacrylamide gel electrophoresis to detect DNA polymorphisms was developed and SSCPs were found to be allelic variants of true Mendelian traits, and therefore they should be useful genetic markers.

TL;DR: It is found that most single base changes in up to 200-base fragments could be detected as mobility shifts and the interspersed repetitive sequences of human, Alu repeats are highly polymorphic.

TL;DR: Results suggested that aberrations of the p53 gene were not correlated with the malignancy of some types of brain tumors such as anaplastic astrocytoma and glioblastoma, contrary to previous observations on colorectal cancers.

TL;DR: This region contains a single confirmed gene, TSLC1, whose expression is reduced or absent in A549 and several other NSCLC, hepatocellular carcinoma (HCC) and pancreatic cancer (PaC) cell lines, and is correlated with promoter methylation state in these cell lines.

TL;DR: The results indicate that the loss of a region of chromosome 13q including the RB1 locus significantly correlates with loss of RB protein in hepatocellular carcinomas, however, tumor-specific mutations of theRB1 gene were detected in only two of 13 tumors with LOH and/or lack of RBprotein expression, indicating that analysis of the RB 1 status at the protein level in these tumors may be more sensitive than the actual mutational analysis.