Characterization of Two Species of Methionine Transfer Ribonucleic Acid from Bakers' Yeast
TLDR
Evidence is provided that yeast N-formylmethionyl-tRNA I serves as an initiator of protein synthesis programmed with the natural messenger in an E. coli system in vitro.About:Â
This article is published in Journal of Biological Chemistry.The article was published on 1968-11-10 and is currently open access. It has received 81 citations till now. The article focuses on the topics: Methionine & Transfer RNA.read more
Citations
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Journal ArticleDOI
Initiation of Haemoglobin Synthesis by Methionyl-tRNA
TL;DR: The synthesis of haemoglobin in a cell free system containing yeast tRNA is initiated by a specific methionine tRNA, which responds to internal codons in the messenger.
Journal ArticleDOI
Cytoplasmic methionine transfer RNAs from eukaryotes.
A. E. Smith,K. A. Marcker +1 more
TL;DR: Eukaryotic cells contain two species of methionine accepting tRNA, and it is suggested that tRNAF*Met is an initiator tRNA on 80S ribosomes.
Book ChapterDOI
Recognition of tRNAs by aminoacyl-tRNA synthetases.
TL;DR: This chapter describes the recognition of tRNAs by aminoacyl-tRNA synthetases, and the roles of the anticodon in recognition, role of the acceptor Stem and discriminator base, and role of modified bases are discussed.
Journal ArticleDOI
Studies on polynucleotides. XCI. Yeast methionine transfer ribonucleic acid: purification, properties, and terminal nucleotide sequences.
U. L. RajBhandary,Hara P. Ghosh +1 more
TL;DR: Methionine tRNA (tRNAfmet) (Peak I) has been purified to homogeneity by ion exchange chromatography on DEAE-Sephadex and by reverse phase chromatography and has the following properties: aminoacylated with methionine by either crude yeast or Escherichia coli aminoacyl tRNA synthetases.
Journal ArticleDOI
Domains of initiator tRNA and initiation codon crucial for initiator tRNA selection by Escherichia coli IF3.
TL;DR: IF3 allows the selection of an initiator tRNA anticodon stem and loop fragment on GUG and UUG codons but does not select that tRNA fragment in response to AUU.
References
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Journal ArticleDOI
Rna codewords and protein synthesis. the effect of trinucleotides upon the binding of srna to ribosomes.
TL;DR: Property of the codeword recognition process and the minimum oligonucleotide chain length required to induce such interactions are presented, and the modification of RNA and DNA codewords, converting sense into missense or nonsense codewording, is suggested as a possible regulatory mechanism in protein synthesis.
Journal ArticleDOI
The role of N-formyl-methionyl-sRNA in protein biosynthesis
B. F. C. Clark,K. A. Marcker +1 more
TL;DR: Bacterial sRNA has been fractionated into two methionine-accepting species, only one of which could be formylated, and the analysis of this polypeptide product identified methionines in internal positions.
Journal ArticleDOI
Studies on polynucleotides
TL;DR: In the in vitro amino acid incorporation experiments, however, no polyaspartate synthesis has so far been detected in the presence of poly AAG, and the new codon sequences ApApG for lysine and GpApA for glutamic acid are proposed.
Journal ArticleDOI
Formyltetrahydrofolate synthetase. I. Isolation and crystallization of the enzyme.
Journal ArticleDOI
Purification of methionine-, valine-, phenylalanine- and tyrosine-specific tRNA from Escherichia coli.
TL;DR: It has been concluded that these amino acid-specific tRNA's are pure enough to use for structural study, suggesting that methionine-tRNA formed a more rigid secondary structure than other t RNA's.
Related Papers (5)
Initiation of Haemoglobin Synthesis by Methionyl-tRNA
N-formylmethionyl transfer RNA in mitochondria from yeast and rat liver
A.E. Smith,K. A. Marcker +1 more