Kok Hao Chen

TL;DR: This report reports multiplexed error-robust FISH (MERFISH), a single-molecule imaging method that allows thousands of RNA species to be imaged in single cells by using combinatorial FISH labeling with encoding schemes capable of detecting and/or correcting errors.

TL;DR: This analysis quantitatively characterized the switching properties of 26 organic dyes and directly related these properties to the quality of super-resolution images, providing guidelines for characterization ofsuper-resolution probes and a resource for selecting probes based on performance.

TL;DR: The results demonstrate that unbiased single-cell RNA–seq profiling of tumor and matched normal samples provides a unique opportunity to characterize aberrant cell states within a tumor.

TL;DR: Advances in multiplexed error-robust fluorescence in situ hybridization (MERFISH) are reported that increase the measurement throughput by two orders of magnitude and allow gene expression profiling of ∼40,000 human cells in a single 18-h measurement, substantially extending the range of biological questions that can be addressed by MERFISH.

TL;DR: It is demonstrated that aptamer-capped near-infrared PbS quantum dots (QDs) can detect a target protein based on selective charge transfer even in the presence of high background concentrations of interfering negatively or positively charged proteins.