LeAnn M. Tiede-Lewis

TL;DR: This work shows that osteocytes, like osteoclasts, are resistant to the negative effects of acid on viability and proposes that the observed reduction in GFP fluorescence in lactating mice is due to quenching of GFP by the acidic pH generated by osteocytes.

TL;DR: Two reproducible methods for 2D and 3D imaging of osteocytes in situ using multiplexed imaging approaches in which the osteocyte cell membrane, nucleus, cytoskeleton and extracellular matrix can be imaged simultaneously in various combinations are reported.

TL;DR: A more complete understanding of the structure and function of the osteocyte network, its lacunocanalicular system, and the degenerative changes that occur with aging should lead to advances in the understanding of age related bone loss and potentially lead to improved therapies.

TL;DR: It is concluded that Rac1 and Rac2 have critical roles in skeletal metabolism in DKO animals and had a normal response to treatment with once‐daily parathyroid hormone (PTH).

TL;DR: Dual live-cell imaging of collagen and osteocyte dynamics in mineralizing primary calvarial cell cultures showed that Dmp1-Cre/tdTomato turned on in early bone nodule forming regions, demarcated by foci of concentrated GFP-collagen bundles that appeared structurally distinct from the surrounding collagen.