Leeann H. Miner

TL;DR: Initial ultrastructural evaluation via preembedding immunogold techniques revealed substantial cytoplasmic NET immunoreactivity in axon terminals within the prelimbic prefrontal cortex, consistent with postulates of regulated trafficking controlling neurotransmitter clearance.

TL;DR: The distribution of immunogold‐silver labeling for SERT (SERT‐ir) in the rat prelimbic PFC is examined and its ultrastructural spatial relationship to dopamine axons labeled by immunoperoxidase staining for tyrosine hydroxylase (TH‐IR) is described.

TL;DR: This work seeks to establish the feasibility of characterizing the ultrastructure of brain tissue disruption associated with the implantation of carbon fiber voltammetric microelectrodes in rats anesthetized with chloral hydrate.

TL;DR: The finding that the majority of NET‐ir axons exhibited no detectable TH immunoreactivity in the VTA of both species suggests that NE can synaptically regulate DA neurons, although functional interactions are more likely to involve extrasynaptic mechanisms.

TL;DR: This chapter addresses the basic applications of tract-tracing and preembedding immunoperoxidase and immunogold-silver labeling for transmission electron microscopy, focusing primarily on identifying the cellular and subcellular localization of proteins of relevance to neurotransmission and on defining synaptic connectivity within neuronal circuits.