Leonie C. van Dinten

TL;DR: This mutant provides the first evidence that the requirements for arterivirus genome replication and discontinuous mRNA synthesis are, at least partially, different and that these processes may be separated experimentally.

TL;DR: An analysis of mutants showed that the selective blockage of ORF1b protein processing affected different stages of EAV reproduction, and the mutant with the nsp10/11 cleavage site mutation Gln-2837→Pro displayed an unusual phenotype.

TL;DR: It is shown that the N-terminal replicase subunit, nonstructural protein 1 (nsp1), of the nidovirus equine arteritis virus is in fact dispensable for replication but crucial for transcription, thereby coupling Replicase expression and subgenomic mRNA synthesis in an unprecedented manner.

TL;DR: The results support a replicase processing model in which the SP cleaves multiple Glu(Gly/Ser/Ala) sites, and characterize the arterivirus SP as a representative of a novel group of chymotrypsin-like enzymes, the 3C-like serine proteases.

TL;DR: The characterized ATPase and helicase activities of bacterially expressed mutant forms of nsp10 and its human coronavirus 229E ortholog, nsp13, and correlated these in vitro activities with specific virus phenotypes suggest that the ZBD is critically involved in nidovirus replication and transcription by modulating the enzymatic activities of the helicase domain and other, yet unknown, mechanisms.