M
Marieke A. Tijms
Researcher at Leiden University Medical Center
Publications - 4
Citations - 327
Marieke A. Tijms is an academic researcher from Leiden University Medical Center. The author has contributed to research in topics: Subgenomic mRNA & RNA-dependent RNA polymerase. The author has an hindex of 4, co-authored 4 publications receiving 325 citations.
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Journal ArticleDOI
A zinc finger-containing papain-like protease couples subgenomic mRNA synthesis to genome translation in a positive-stranded RNA virus.
TL;DR: It is shown that the N-terminal replicase subunit, nonstructural protein 1 (nsp1), of the nidovirus equine arteritis virus is in fact dispensable for replication but crucial for transcription, thereby coupling Replicase expression and subgenomic mRNA synthesis in an unprecedented manner.
Journal ArticleDOI
Nuclear localization of non-structural protein 1 and nucleocapsid protein of equine arteritis virus
TL;DR: It is revealed that another EAV protein with a partially nuclear localization, the nucleocapsid (N) protein, utilizes the CRM1-mediated nuclear export pathway, and inactivation of this pathway with the drug leptomycin B resulted in the unexpected and immediate nuclear retention of all N protein molecules, thus revealing that the protein shuttles between cytoplasm and nucleus before playing its role in cy toplasmic virus assembly.
Journal ArticleDOI
Arterivirus Subgenomic mRNA Synthesis and Virion Biogenesis Depend on the Multifunctional nsp1 Autoprotease
Marieke A. Tijms,Danny D. Nedialkova,Jessika C. Zevenhoven-Dobbe,Alexander E. Gorbalenya,Eric J. Snijder +4 more
TL;DR: The role of nsp1 in the EAV life cycle has been investigated using reverse genetics in this article, which revealed that Nsp1 is a multifunctional regulatory protein, responsible for replicase polyprotein processing, transcription, and virion biogenesis.
Journal ArticleDOI
Equine arteritis virus non-structural protein 1, an essential factor for viral subgenomic mRNA synthesis, interacts with the cellular transcription co-factor p100.
Marieke A. Tijms,Eric J. Snijder +1 more
TL;DR: The interaction of nsp1 with one of these proteins, p100, a transcription co-activator that also interacts with regulatory proteins of other viruses, was confirmed by mutual co-immunoprecipitation from lysates of EAV-susceptible mammalian cells.