Lesley Mutch

TL;DR: Analysis of differential expression showed that acidophilic microorganisms adopted several changes in their proteomes in the presence of chloride ions, suggesting the following strategies to combat the NaCl stress: adaptation of the cell membrane, the accumulation of amino acids possibly as a form of osmoprotectant, and the expression of a YceI family protein involved in acid and osmotic-related stress.

TL;DR: In this paper, three largely independent studies were undertaken on the same heap leach system during the period of transition from processing oxidised ores to sulfide ores: monitoring of heap solutions for microorganisms, analysis of samples from a spent heap, and column tests.

TL;DR: In this paper, the effect of temperature on the population dynamics of microorganisms in bio-leaching columns charged with a low-grade chalcopyrite ore was investigated, and the results brought into question the effectiveness of heap inoculation, a strategy proposed to overcome the paucity of thermophilic organisms occurring naturally.

TL;DR: A method for the reliable, representative and reproducible extraction of NA from five strains of biomining and AMD microorganisms from pyrite and from liquid culture is described.

TL;DR: This study evaluates the use of Q-PCR for the enumeration of biomining microorganisms in liquid cultures using a standard curve constructed with DNA from Sinorhizobium meliloti and At.