L
Liisa D. Van Vliet
Researcher at University of Cambridge
Publications - 18
Citations - 581
Liisa D. Van Vliet is an academic researcher from University of Cambridge. The author has contributed to research in topics: Chemistry & Microfluidics. The author has an hindex of 10, co-authored 15 publications receiving 476 citations. Previous affiliations of Liisa D. Van Vliet include Central Queensland University.
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Journal ArticleDOI
Microfluidic droplets: new integrated workflows for biological experiments.
TL;DR: The emerging experimental format makes possible a quantitative readout for large numbers of experiments with a precision comparable to the macroscopic scale, which provides the basis for the design of more complex workflows to address biological challenges.
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A fully unsupervised compartment-on-demand platform for precise nanoliter assays of time-dependent steady-state enzyme kinetics and inhibition.
Fabrice Gielen,Liisa D. Van Vliet,Bartosz T. Koprowski,Sean R. A. Devenish,Martin Fischlechner,Joshua B. Edel,Xize Niu,Andrew J. deMello,Florian Hollfelder +8 more
TL;DR: The ability to miniaturize biochemical assays in water-in-oil emulsion droplets allows a massive scale-down of reaction volumes, so that high-throughput experimentation can be performed more economically and more efficiently.
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Molecular recognition of DNA by rigid [N]-polynorbornane-derived bifunctional intercalators: synthesis and evaluation of their binding properties.
Liisa D. Van Vliet,Tom Ellis,Patrick J. Foley,Ligong Liu,Frederick M. Pfeffer,Richard A. Russell,Ronald N. Warrener,Florian Hollfelder,Michael J. Waring +8 more
TL;DR: The concept of multivalency is exploited in the context of DNA recognition, using novel chemistry to synthesize a new type of bis-intercalator with unusual sequence-selectivity and 9-aminoacridine rings were preferred over acridine carboxamides or naphthalimides.
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Polyethylene imine derivatives ('synzymes') accelerate phosphate transfer in the absence of metal
TL;DR: systematic derivatization of polyethylene imine with alkyl (C(2)-C(12)), benzyl, and guanidinium groups gives rise to catalysts ('synzymes') with rate accelerations of up to 10(4) for the intramolecular transesterification of 2-hydroxypropyl-p-nitrophenyl phosphate, HPNP, in the absence of metal.
Journal ArticleDOI
Interfacing microwells with nanoliter compartments: a sampler generating high-resolution concentration gradients for quantitative biochemical analyses in droplets.
Fabrice Gielen,Tomas Buryska,Liisa D. Van Vliet,Maren Butz,Jiri Damborsky,Zbynek Prokop,Florian Hollfelder +6 more
TL;DR: This work addresses the challenge of producing microfluidic-based concentration gradients in a way that every droplet represents one unique reagent combination by presenting a simple microcapillary technique able to generate series of monodisperse water-in-oil droplets from a sample presented in an open well.