L
Lijuan Wu
Researcher at East China Normal University
Publications - 3
Citations - 396
Lijuan Wu is an academic researcher from East China Normal University. The author has contributed to research in topics: Genome editing & Gene. The author has an hindex of 3, co-authored 3 publications receiving 334 citations.
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Journal ArticleDOI
CRISPR/Cas-mediated genome editing in the rat via direct injection of one-cell embryos
Yanjiao Shao,Yuting Guan,Liren Wang,Zhongwei Qiu,Meizhen Liu,Yuting Chen,Lijuan Wu,Yongmei Li,Xueyun Ma,Mingyao Liu,Mingyao Liu,Dali Li +11 more
TL;DR: This protocol provides guidelines for the selection of genomic targets, synthesis of guide RNAs, design and construction of homologous recombination template vectors, embryo microinjection, and detection of mutations and insertions in founders or their progeny.
Journal ArticleDOI
CRISPR/Cas9‐mediated somatic correction of a novel coagulator factor IX gene mutation ameliorates hemophilia in mouse
Yuting Guan,Yanlin Ma,Yanlin Ma,Qi Li,Zhenliang Sun,Lie Ma,Lijuan Wu,Liren Wang,Li Zeng,Yanjiao Shao,Yuting Chen,Ning Ma,Wenqing Lu,Kewen Hu,Honghui Han,Yanhong Yu,Yuanhua Huang,Mingyao Liu,Mingyao Liu,Dali Li +19 more
TL;DR: The studies suggest that CRISPR/Cas‐mediated in situ genome editing could be a feasible therapeutic strategy for human hereditary diseases, although an efficient and clinically relevant delivery system is required for further clinical studies.
Journal ArticleDOI
Large genomic fragment deletion and functional gene cassette knock-in via Cas9 protein mediated genome editing in one-cell rodent embryos
Liren Wang,Yanjiao Shao,Yuting Guan,Liang Li,Lijuan Wu,Fangrui Chen,Meizhen Liu,Huaqing Chen,Yanlin Ma,Xueyun Ma,Mingyao Liu,Mingyao Liu,Dali Li +12 more
TL;DR: Through the injection of Cas9 protein instead of mRNA into embryos, the CRISPR-Cas RNA-guided system is used for novel genetically modified mouse and rat models and fewer off-target effects of Cas 9 are observed and point mutation knock-in efficiency is increased.