Lu Wei

TL;DR: In this paper, the authors used stimulated Raman-scattering imaging of alkyne tags as a general strategy for studying a broad spectrum of small biomolecules in live cells and animals.

TL;DR: Bioorthogonal chemical imaging of DNA, RNA, protein and lipid metabolism in live rat brain hippocampal tissues is demonstrated by coupling stimulated Raman scattering microscopy with integrated deuterium and alkyne labeling and paves the way for the study of complex metabolic profiles in live brain tissue under both physiological and pathological conditions with single-cell resolution and minimal perturbation.

TL;DR: Proof-of-principle experiments on neuronal co-cultures and brain tissues reveal cell-type dependent heterogeneities in DNA and protein metabolism under physiological and pathological conditions, underscoring the potential of this super-multiplex optical imaging approach for untangling intricate interactions in complex biological systems.

TL;DR: These polyynes hold great promise in live-cell imaging and sorting as well as in high-throughput diagnostics and screening, and the largest number of distinct spectral barcodes to date is realized.

TL;DR: An imaging technique to visualize nascent proteins by harnessing the emerging stimulated Raman scattering (SRS) microscopy coupled with metabolic incorporation of deuterium-labeled amino acids is reported, which can readily generate spatial maps of the quantitative ratio between new and total proteomes.